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Product Info Summary
| SKU: | EK1686 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human ULBP3 ELISA Kit PicoKine®
SKU/Catalog Number
EK1686
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ULBP3 ELISA Kit PicoKine® (96 Tests). Quantitate Human ULBP3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ULBP3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1686)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D30-G217
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1686 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1686 is reactive to ULBP3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1686 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ULBP-3
UL16 binding protein 3 (ULBP3) is a cell surface glycoprotein encoded by ULBP3 gene located on the chromosome 6. It is mapped to 6q25.1. ULBP3 is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the α3 domain and the transmembrane segment. ULBP3 is thus composed of only the α1α2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor. The protein encoded by this gene is one of several related ligands of the KLRK1/NKG2D receptor, which is found in primary NK cells. Binding of these ligands to the receptor activates several signal transduction pathways, including the JAK2, STAT5, and ERK pathways. The encoded protein is expressed solubly and on the surface of many tumor cells, making it potentially an important target for therapeutics.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1686-CAP | Anti-Human ULBP3 Pre-coated 96-well strip microplate | 1 |
| EK1686-ST | Human ULBP3 Standard | 2 vials, 10 ng/tube |
| EK1686-DA | Human ULBP3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.148 | 0.189 | 0.228 | 0.319 | 0.573 | 0.839 | 1.384 | 2.077 |
Data Example Images
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Human ULBP3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ULBP-3, Dilution ratio of 1:1, concentration in serum and plasma is around 260 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ULBP3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 98 | 477 | 1133 | 101 | 482 | 1425 |
| Standard deviation | 3.92 | 20.51 | 52.12 | 5.15 | 26.99 | 84.08 |
| CV (%) | 4.0% | 4.3% | 4.6% | 5.1% | 5.6% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ULBP-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 98 | 98 | 102 | 85 | 95 | 6.41 | 6.7% |
| Sample 2 | 477 | 459 | 496 | 467 | 474 | 13.82 | 2.9% |
| Sample 3 | 1133 | 1050 | 1116 | 1070 | 1092 | 33.55 | 3.0% |
Protein Target Info & Infographic
Gene/Protein Information For ULBP3 (Source: Uniprot.Org, NCBI)
Gene Name
ULBP3
Full Name
UL16-binding protein 3
Weight
27.949kDa
Superfamily
MHC class I family
Alternative Names
UL16-binding protein 3; ALCAN-gamma; NKG2D ligand 3; N2DL-3; NKG2DL3; Retinoic acid early transcript 1N; ULBP3; N2DL3; RAET1N ULBP3 N2DL-3, NKG2DL3, RAET1N UL16 binding protein 3 UL16-binding protein 3|ALCAN-gamma|NKG2D ligand 3|retinoic acid early transcript 1N
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ULBP3, check out the ULBP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ULBP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ULBP3 ELISA Kit PicoKine® (EK1686)
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Customer Q&As
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7 Customer Q&As for Human ULBP3 ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis ULBP-3 using the EK1686 Human ULBP-3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-09-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-09-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
B. Patel
Verified customer
Asked: 2020-09-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-18
Question
Q: can I use citrate plasma as samples in Human ULBP-3 Picokine® ELISA Kit (Catalog # EK1686)?
Verified Customer
Verified customer
Asked: 2020-05-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of ULBP-3 causing degradation of its protein structure. ULBP-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the ULBP-3 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-26
Question
Q: how do I analyze ELISA data? I have performed ULBP-3 level in plasma.
Verified Customer
Verified customer
Asked: 2020-05-17
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-05-17
Question
Q: if the enzyme conjugated ULBP-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ULBP-3 antigen?
T. Dhawan
Verified customer
Asked: 2019-12-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-14
Question
Q: What is the optimal O.D. value for ULBP-3 ELISA kit? I performed your ULBP-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ULBP-3 even though the O.D. values are not very high?
T. Thomas
Verified customer
Asked: 2019-02-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ULBP-3 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of ULBP-3 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2019-02-08
Question
Q: how can I thaw whole blood sample for ULBP-3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-01-20
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ULBP-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-20
