Click image to see more details
Product Info Summary
| SKU: | EK0659 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human AXL ELISA Kit PicoKine®
SKU/Catalog Number
EK0659
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human AXL ELISA Kit PicoKine™ (96 Tests). Quantitate Human AXL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human AXL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0659)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E33-P440
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0659 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0659 is reactive to AXL in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0659 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of AXL
Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0659-CAP | Anti-Human AXL Pre-coated 96-well strip microplate | 1 |
| EK0659-ST | Human AXL Standard | 2 vials, 10 ng/tube |
| EK0659-DA | Human AXL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
| O.D. | 0.063 | 0.09 | 0.135 | 0.199 | 0.345 | 0.655 | 1.155 | 2.066 |
Data Example Images
Click image to see more details
Human AXL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect AXL, For serum, dilution ratio is 1:20 and concentration is 4-39 ng/ml.
For plasma, dilution ratio is 1:20 and concentration is 17-66 ng/ml..
Some articles we found to cite concentrations of AXL in samples: 24239956 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human AXL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 103 | 593 | 12107 | 107 | 550 | 1194 |
| Standard deviation | 8.13 | 45.06 | 86.76 | 9.3 | 49.5 | 86.7%6 |
| CV (%) | 7.9% | 7.6% | 6.7% | 8.7% | 9% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of AXL in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 103 | 88 | 85 | 85 | 90 | 7.46 | 8.2% |
| Sample 2 | 593 | 706 | 654 | 601 | 638 | 45.47 | 7.1% |
| Sample 3 | 1295 | 1325 | 1154 | 1246 | 1255 | 64.77 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For AXL (Source: Uniprot.Org, NCBI)
Gene Name
AXL
Full Name
Tyrosine-protein kinase receptor UFO
Weight
98336 MW
Superfamily
protein kinase superfamily
Alternative Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL AXL ARK, JTK11, Tyro7, UFO AXL receptor tyrosine kinase tyrosine-protein kinase receptor UFO|AXL oncogene|AXL transforming sequence/gene
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on AXL, check out the AXL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for AXL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human AXL ELISA Kit PicoKine® (EK0659)
Hello CJ!
No publications found for EK0659
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human AXL ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human AXL ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Human AXL ELISA Kit PicoKine®
Question
Could EK0659 detect cAXL in tumor, and sAXL and pAXL in serum or plasma?
Verified customer
Asked: 2022-05-10
Answer
The Human AXL ELISA Kit PicoKine® (EK0659) can detect soluble AXL (sAXL) in serum or plasma. Composite AXL (cAXL) in tumor and Phosphorylated Axl (pAXL) hasn't been validated with EK0695.
Boster Scientific Support
Answered: 2022-05-11
Question
Q: if the enzyme conjugated AXL antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the AXL antigen?
K. Young
Verified customer
Asked: 2019-10-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-10-26
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-10-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-04-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-21
Question
Q: which procedure should I follow in order to thaw whole blood sample for AXL ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test AXL for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-04-02
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of AXL in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
W. Mangal
Verified customer
Asked: 2018-03-06
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the AXL ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-06
