Product Info Summary
SKU: | A01568-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Protein S/PROS1 Antibody Picoband®
View all Protein S/PROS1 Antibodies
SKU/Catalog Number
A01568-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein S/PROS1 Antibody Picoband® catalog # A01568-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein S/PROS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01568-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Protein S recombinant protein (Position: L291-S676).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01568-1 is reactive to PROS1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
28681 MW
Background of Protein S/PROS1
This PROS1 gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01568-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Protein S using anti-Protein S antibody (A01568-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein S antigen affinity purified polyclonal antibody (Catalog # A01568-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein S at approximately 75KD. The expected band size for Protein S is at 75KD.
Protein Target Info & Infographic
Gene/Protein Information For PROS1 (Source: Uniprot.org, NCBI)
Gene Name
PROS1
Full Name
Vitamin K-dependent protein S
Weight
28681 MW
Alternative Names
Vitamin K-dependent protein S; PROS1; PROS PROS1 PROS, PS21, PS22, PS23, PS24, PS25, PSA, THPH5, THPH6 protein S vitamin K-dependent protein S|protein S (alpha)|protein Sa|vitamin K-dependent plasma protein S
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROS1, check out the PROS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein S/PROS1 Antibody Picoband® (A01568-1)
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Customer Q&As
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4 Customer Q&As for Anti-Protein S/PROS1 Antibody Picoband®
Question
I was wanting to use your anti-Protein S/PROS1 antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
As indicated on the product datasheet, A01568-1 anti-Protein S/PROS1 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-10
Question
I see that the anti-Protein S/PROS1 antibody A01568-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-30
Question
Would anti-Protein S/PROS1 antibody A01568-1 work for WB with uterus?
Verified Customer
Verified customer
Asked: 2017-06-07
Answer
According to the expression profile of uterus, PROS1 is highly expressed in uterus. So, it is likely that anti-Protein S/PROS1 antibody A01568-1 will work for WB with uterus.
Boster Scientific Support
Answered: 2017-06-07
Question
We are currently using anti-Protein S/PROS1 antibody A01568-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
D. Walker
Verified customer
Asked: 2015-02-17
Answer
The anti-Protein S/PROS1 antibody (A01568-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-17