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Product Info Summary
| SKU: | FEK0905 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0905
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human PLG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0905)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V98-S355 (K1-3)
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0905 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0905 is reactive to PLG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0905 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PLG
Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (EDâ‚…â‚€ =70 nM) relative to kringles 1-4 (EDâ‚…â‚€ = 135 nM).
Kit Components
| Catalog number | Description | Quantity |
|---|---|---|
| FEK0905-CAP | 96-well plate precoated with anti-Human PLG antibody | 1 |
| EK0905-ST | lyophilized recombinant Human PLG standard | 100 ng/tube |
| FEK0905-DA | Biotinylated anti-Human PLG detection antibody (50x) | 100 ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30 ml |
| AR1106-2 | Antibody diluent | 12 ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12 ml |
| AR1106-5 | Wash Buffer (25x) | 20 ml |
| AR1104 | Color Developing Reagent (TMB) | 10 ml |
| AR1105 | Stop Solution | 10 ml |
| PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.024 | 0.137 | 0.201 | 0.426 | 0.968 | 1.338 | 1.568 | 2.094 |
Data Example Images
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Human Angiostatin K1-3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 241 | 1987 | 5716 | 218 | 1908 | 5845 |
| Standard deviation | 12.05 | 79.48 | 337.24 | 13.73 | 91.58 | 432.53 |
| CV (%) | 5% | 4% | 5.9% | 4.8%% | 4.8% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PLG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 241 | 249 | 248 | 219 | 239 | 12.09 | 5% |
| Sample 2 | 1987 | 1892 | 1804 | 1733 | 1854 | 95.22 | 5.1% |
| Sample 3 | 5716 | 5780 | 5230 | 5301 | 5506 | 243.6 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For PLG (Source: Uniprot.Org, NCBI)
Uniprot ID
P00747
Gene ID
5340
Gene Name
PLG
Full Name
Plasminogen
Weight
90569 MW
Superfamily
peptidase S1 family
Alternative Names
Plasminogen;3.4.21.7;Plasmin heavy chain A;Activation peptide;Angiostatin;Plasmin heavy chain A, short form;Plasmin light chain B;PLG; PLG plasminogen plasminogen|plasmin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PLG, check out the PLG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit (FEK0905)
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