FGF-2 Fibroblast Growth Factor-basic Rat Recombinant Protein

Product Name

FGF-2 Fibroblast Growth Factor-basic Rat Recombinant Protein

View all FGF basic/FGF2/bFGF recombinant proteins

SKU/Catalog Number

PROTP13109

Size

10ug, 50ug, 1mg

Description

bFGF Rat Recombinant (FGF-2) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.4 kDa. The bFGF2 is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Rat bFGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat FGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

FGF-2 Fibroblast Growth Factor-basic Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP13109)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

FGF-b was lyophilized from 1mg/ml solution after extensive dialysis against 10mM Na₂PO₄, pH 7.5 and 50mM NaCl.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Predicted MW

30.77kDa

Reconstitution

It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀ range as determined by the dose-dependant proliferation of NR6R-3T3 cells was found to be 0.903ng/ml, corresponding to a specific activity of 1.1x10⁷ units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For FGF2 (Source: Uniprot.org, NCBI)

Alternative Names

HBGH-2; HBGF-2; Prostatropin; FGF-2; FGB-b; Fibroblast Growth Factor-basic; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2 FGF2 BFGF, FGF-2, FGFB, HBGF-2 fibroblast growth factor 2 fibroblast growth factor 2|basic fibroblast growth factor bFGF|fibroblast growth factor 2 (basic)|heparin-binding growth factor 2|prostatropin

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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