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Product Info Summary
| SKU: | EKC2404 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit
See all ER alpha/NR3A1 products
SKU/Catalog Number
EKC2404
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Estrogen Receptor-alpha (Phospho-Ser167) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Estrogen Receptor-alpha (Phospho-Ser167) protein expression profile in cells. The kit can be used for measuring the relative amounts of Estrogen Receptor-alpha (Phospho-Ser167) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Estrogen Receptor-alpha (Phospho-Ser167).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2404)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2404 is reactive to ESR1 in Human samples
Application Guarantee
EKC2404 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Estrogen Receptor-alpha Phospho-Ser167 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Estrogen Receptor-alpha Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from MCF7 cells treated with EGF
Protein Target Info & Infographic
Gene/Protein Information For ESR1 (Source: Uniprot.Org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66216 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1;ESR1;ESR, NR3A1; ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ESR1, check out the ESR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ESR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit (EKC2404)
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Customer Q&As
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2 Customer Q&As for Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit
Question
I purchased your product Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit (EKC2404) and performed Western blot assay but the results shows multiple bands and wrong band size. I need your help to determine the correct size of Western blot image.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We understand that you expect to see the receptor at 65kDA but you did not. These are the few things we want to ask; what are the samples you used? How did you prepare RIPA lysis buffer? Are they collected in protease inhibitors? Did you boil the sample to denature with the reducing agent? How are the membranes blocked? What is the dilution or concentration of the antibody? What are the sizes (molecular weights) of the bands that you observed? Looking forward your reply to resolve the issue.
Boster Scientific Support
Answered: 2019-08-22
Question
To Support: Our results was not good on FFPE sections. Could you provide us any information about Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit concentration and type of antigen retrieval used for the beautiful staining images?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
We recommend a dilution range in IHC-P is 1:20 to 1:80 and this Estrogen Receptor-alpha (Phospho-Ser167) Colorimetric Cell-Based ELISA Kit requires antigen retrieval with citrate buffer pH 6.0.
Boster Scientific Support
Answered: 2017-09-01
