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Product Info Summary
| SKU: | EK1114 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Granzyme B ELISA Kit PicoKine®
View all Granzyme B ELISA kits
SKU/Catalog Number
EK1114
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Granzyme B ELISA Kit PicoKine® (96 Tests). Quantitate Human GZMB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Granzyme B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1114)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: I21-Y247
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1114 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1114 is reactive to GZMB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1114 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Granzyme B
Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1114-CAP | Anti-Human GZMB Pre-coated 96-well strip microplate | 1 |
| EK1114-ST | Human GZMB Standard | 2 vials, 10 ng/tube |
| EK1114-DA | Human GZMB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.065 | 0.109 | 0.156 | 0.308 | 0.590 | 1.019 | 1.863 |
Data Example Images
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Human Granzyme B PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Granzyme B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 35 | 170 | 476 | 38 | 176 | 487 |
| Standard deviation | 2.73 | 7.14 | 27.6 | 3.26 | 10.2 | 32.62 |
| CV (%) | 7.8% | 4.2% | 5.8% | 8.6% | 5.8% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Granzyme B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 36 | 36 | 35 | 35 | 0.5 | 1.4% |
| Sample 2 | 170 | 190 | 185 | 175 | 180 | 7.9 | 4.3% |
| Sample 3 | 476 | 469 | 464 | 420 | 457 | 21.92 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For GZMB (Source: Uniprot.Org, NCBI)
Gene Name
GZMB
Full Name
Granzyme B
Weight
27716 MW
Superfamily
peptidase S1 family
Alternative Names
Granzyme B;3.4.21.79;C11;CTLA-1;Cathepsin G-like 1;CTSGL1;Cytotoxic T-lymphocyte proteinase 2;Lymphocyte protease;Fragmentin-2;Granzyme-2;Human lymphocyte protein;HLP;SECT;T-cell serine protease 1-3E;GZMB;CGL1, CSPB, CTLA1, GRB; GZMB C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT granzyme B granzyme B|T-cell serine protease 1-3E|cathepsin G-like 1|cytotoxic T-lymphocyte proteinase 2|cytotoxic serine protease B|fragmentin 2|granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)|human lymphocyte protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GZMB, check out the GZMB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GZMB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Granzyme B ELISA Kit PicoKine® (EK1114)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Human Granzyme B ELISA Kit PicoKine®
Question
Is EK1114 suitable for co-culture Target Cells and Effector Cells and ELISA with cell culture fluid?
Verified customer
Asked: 2020-11-12
Answer
Our lab hasn't worked on co-culture cell samples using the Human Granzyme B ELISA Kit PicoKine™ (EK1114). Please run a pilot test to see if the kits could work on the specific samples.
Boster Scientific Support
Answered: 2020-11-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
L. Green
Verified customer
Asked: 2020-07-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-30
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
M. Wilson
Verified customer
Asked: 2020-02-28
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-02-28
Question
I would like to know the species of the capture and detection antibodies for this product EK1114 and what is the concentration of the detection antibody?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The product Human Granzyme B ELISA Kit are raised in mouse. The capture antibody coated into the well is (Fab)' 2 IgG1, while the biotinylated detection antibody tracer is whole IgG1. For the concentration it is in the range of 1- 0.5
Boster Scientific Support
Answered: 2020-02-18
Question
Q: What is the optimal O.D. value for Granzyme B ELISA kit? I used your Granzyme B ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Granzyme B even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-02-08
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Granzyme B ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Granzyme B that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-08
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Granzyme B in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Granzyme B ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-02-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-01-18
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-01-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Wright
Verified customer
Asked: 2019-09-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-01
Question
Q: if the enzyme conjugated Granzyme B antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Granzyme B antigen?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-12-13
Question
Q: can I use citrate plasma as samples in Human Granzyme B Picokine® ELISA Kit (Catalog # EK1114)?
Verified Customer
Verified customer
Asked: 2018-10-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Granzyme B causing disruption of its protein structure. Granzyme B may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Granzyme B ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-28
Question
Q: how can I thaw whole blood sample for Granzyme B ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-07-14
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Granzyme B for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-07-14
