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Product Info Summary
| SKU: | A06292-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Zinc finger protein 521 ZNF521 Antibody
SKU/Catalog Number
A06292-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Zinc finger protein 521 ZNF521 Antibody (Catalog # A06292-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
ZNF521 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Zinc finger protein 521 ZNF521 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06292-1)
Host
Rabbit
Contents
ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521. The immunogen is located within the first 50 amino acids of ZNF521.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ZNF521 antibody is human specific.
Reactive Species
A06292-1 is reactive to ZNF521 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
147866 MW
Background of ZNF521
The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06292-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZNF521 antibody can be used for detection of ZNF521 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ZNF521 in HeLa cells with ZNF521 antibody at 2.5 μg/mL.
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Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZNF521 (Source: Uniprot.org, NCBI)
Gene Name
ZNF521
Full Name
Zinc finger protein 521
Weight
147866 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
EHZF, Evi3, EHZF, LIP3, Zinc finger protein 521, Early hematopoietic zinc finger protein ZNF521 EHZF, Evi3 zinc finger protein 521 zinc finger protein 521|LYST-interacting protein 3|early hematopoietic zinc finger protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNF521, check out the ZNF521 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNF521: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zinc finger protein 521 ZNF521 Antibody (A06292-1)
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