Click image to see more details
Product Info Summary
| SKU: | M00270 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) VWF Monoclonal Antibody
SKU/Catalog Number
M00270
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) VWF Monoclonal Antibody (Catalog # M00270). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) VWF Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00270)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM577
Isotype
IgG1, kappa
Immunogen
Recombinant human vWF fragment
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00270 is reactive to VWF in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
309265 MW
Background of VWF
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00270 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human tonsil stained with Anti-vWF Mouse Monoclonal Antibody (SPM577).
Protein Target Info & Infographic
Gene/Protein Information For VWF (Source: Uniprot.org, NCBI)
Gene Name
VWF
Full Name
von Willebrand factor
Weight
309265 MW
Alternative Names
von Willebrand factor;vWF;von Willebrand antigen 2;von Willebrand antigen II;VWF;F8VWF; VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VWF, check out the VWF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VWF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) VWF Monoclonal Antibody (M00270)
Hello CJ!
M00270 has been cited in 15 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Application of bFGF and BDNF to Improve Angiogenesis and Cardiac Function
Li X,Zhou J,Dou Y,Shi Y,Wang Y,Hong J,Zhao J,Zhang J,Yuan Y,Zhou M,Wei X.The protective effects of angelica organic acid against ox-LDL-induced autophagy dysfunction of HUVECs.BMC Complement Med Ther.2020 Jun 1;20(1):164.doi:10.1186/s12906-020-02968-7.PMID:32487223;PMCID:PMC7268640.
Species: Human
M00270 usage in article: APP:IF, SAMPLE:HUVECS, DILUTION:1:50
Wang B,Lv X,Li Z,Zhang M,Yao J,Sheng N,Lu M,Wang H,Chen S.Urethra-inspired biomimetic scaffold: A therapeutic strategy to promote angiogenesis for urethral regeneration in a rabbit model.Acta Biomater.2020 Jan 15;102:247-258.doi:10.1016/j.actbio.2019.11.0
Species: Human,New Zealand Rabbit
M00270 usage in article: APP:IHC, SAMPLE:HUVECS, DILUTION:1:500
Liu H,Chen W,Zhao B,Quan W,Zhang Y,Zhou Y,Wan Z,Zhang X,Xue G,Li J,Luo S,Wang J,Liu Y,Zhen M,Zhao Y.Autologous bionic tissue for inguinal hernia repair.J Biomed Mater Res A.2020 Jun; 108(6):1351-1368.doi:10.1002/jbm.a.36907.Epub 2020 Mar 2.PMID:32090432.
Species: New Zealand white Rabbit
M00270 usage in article: APP:IF, SAMPLE:MSCS, DILUTION:NA
Homocysteine induced oxidative stress in human umbilical vein endothelial cells via regulating methylation of SORBS1.
Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury
Resveratrol Protects against TNF-?-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK
IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells
Paxillin regulates vascular endothelial growth factor A-induced?in vitro?angiogenesis of human umbilical vein endothelial cells
Yan J, Wang J, Huang H, Huang Y, Mi T, Zhang C, Zhang L. Am J Transl Res. 2017 Oct 15;9(10):4492-4501. eCollection 2017. Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senesce...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) VWF Monoclonal Antibody
Question
Is a blocking peptide available for product anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody (M00270)?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
We do provide the blocking peptide for product anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody (M00270). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-04
Question
We have observed staining in human platelet. Do you have any suggestions? Is anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
According to literature platelet does express VWF. According to Uniprot.org, VWF is expressed in lung, umbilical vein endothelial cell, platelet, plasma, liver, among other tissues. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2020-05-01
Question
I see that the anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-11
Question
Can you help my question with product M00270, anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00270 anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-03
Question
We need to test anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 on human liver for research purposes, then I may be interested in using anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
Does M00270 anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, M00270 anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-11
Question
Is this M00270 anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody reactive to the isotypes of VWF?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The immunogen of M00270 anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody is Recombinant human vWF fragment. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-07
Question
Our lab were well pleased with the WB result of your anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody. However we have observed positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is VWF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
From what I have seen in literature, liver does express VWF. Generally VWF expresses in secreted. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
It shows on the product datasheet, M00270 anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Is there a BSA free version of anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 available?
N. Rodriguez
Verified customer
Asked: 2019-05-21
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-21
Question
Does anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 work for IHC with liver?
C. Johnson
Verified customer
Asked: 2017-05-29
Answer
According to the expression profile of liver, VWF is highly expressed in liver. So, it is likely that anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 will work for IHC with liver.
Boster Scientific Support
Answered: 2017-05-29
Question
We are currently using anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
T. Carter
Verified customer
Asked: 2015-07-20
Answer
The anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody (M00270) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270. Let me know if you need anything else.
V. Patel
Verified customer
Asked: 2014-03-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-17
Question
I have attached the WB image, lot number and protocol we used for liver using anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal antibody M00270. Please let me know if you require anything else.
O. Bhatt
Verified customer
Asked: 2013-09-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-10
