Anti-Factor VII/F7 Antibody Picoband®

Product Name

Anti-Factor VII/F7 Antibody Picoband®

View all Coagulation Factor VII Antibodies

SKU/Catalog Number

PA2060

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Factor VII/F7 Antibody catalog # PA2060. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Factor VII/F7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2060)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2060 is reactive to F7 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

52 kDa

Calculated molecular weight

51594 MW

Background of Coagulation Factor VII

F7 (Coagulation Factor VII), also known as proconvertin, is one of the proteins that causes blood to clot in the coagulation cascade. It is an enzyme of the serine protease class. The F7 gene maps to chromosome 13q34 (Millar et al., 2000). Synthesis of factors VII and X, as well as factors II and IX, takes place in the liver and requires vitamin K. Structural homologies of these factors, which are precursors of serine proteases, have been shown (Zur and Nemerson, 1981). Di Bitondo et al. (2002) used reporter gene analysis to show that inclusion of promoter regions of F7 reduced transcription activity in the presence of estrogenic factors. The effect was independent of promoter polymorphic haplotype.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2060 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: SMMC Whole Cell, JURKAT Whole Cell, RAJI Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For F7 (Source: Uniprot.org, NCBI)

Alternative Names

Coagulation factor VII;3.4.21.21;Proconvertin;Serum prothrombin conversion accelerator;SPCA;Eptacog alfa;Factor VII light chain;Factor VII heavy chain;F7; F7 SPCA coagulation factor VII coagulation factor VII|FVII coagulation protein|coagulation factor VII (serum prothrombin conversion accelerator)|eptacog alfa|proconvertin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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