Product Info Summary
SKU: | A00901-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-VEGF Receptor 2/KDR Antibody Picoband™
View all VEGFR2/KDR/Flk-1 Antibodies
SKU/Catalog Number
A00901-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF Receptor 2/KDR Antibody Picoband™ catalog # A00901-2. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF Receptor 2/KDR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00901-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VEGF Receptor 2 recombinant protein (Position: A20-L242).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00901-2 is reactive to KDR in Human
Applications
A00901-2 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
180-250 kDa
Calculated molecular weight
78265 MW
Background of VEGFR2/KDR/Flk-1
KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells. Flk1 is the mouse homolog of KDR.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
ELISA (Cap),1-5μg/ml
Validation Images & Assay Conditions
![a00901 2 vegfr2 primary antibodies wb testing 1 a00901 2 vegfr2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00901-2-vegfr2-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 2 at approximately 180-250 kDa. The expected band size for VEGF Receptor 2 is at 156 kDa.
![A00901 2 VEGF Receptor 2 primary antibodies IHC testing 2 A00901 2 VEGF Receptor 2 primary antibodies IHC testing 2](https://www.bosterbio.com/media/catalog/product/A/0/A00901-2-VEGF-Receptor-2--primary-antibodies-IHC-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2).
VEGF Receptor 2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-2) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A00901 2 VEGF Receptor 2 primary antibodies IHC testing 3 A00901 2 VEGF Receptor 2 primary antibodies IHC testing 3](https://www.bosterbio.com/media/catalog/product/A/0/A00901-2-VEGF-Receptor-2--primary-antibodies-IHC-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2).
VEGF Receptor 2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-2) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A00901 2 VEGF Receptor 2 primary antibodies IHC testing 4 A00901 2 VEGF Receptor 2 primary antibodies IHC testing 4](https://www.bosterbio.com/media/catalog/product/A/0/A00901-2-VEGF-Receptor-2--primary-antibodies-IHC-testing-4.jpg)
Click image to see more details
Figure 4. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2).
VEGF Receptor 2 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-2) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A00901 2 VEGF Receptor 2 primary antibodies IHC testing 5 A00901 2 VEGF Receptor 2 primary antibodies IHC testing 5](https://www.bosterbio.com/media/catalog/product/A/0/A00901-2-VEGF-Receptor-2--primary-antibodies-IHC-testing-5.jpg)
Click image to see more details
Figure 5. IHC analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-2).
VEGF Receptor 2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 2 Antibody (A00901-2) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KDR (Source: Uniprot.org, NCBI)
Gene Name
KDR
Full Name
Vascular endothelial growth factor receptor 2
Weight
78265 MW
Superfamily
protein kinase superfamily
Alternative Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDR, check out the KDR Infographic
![KDR infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGF Receptor 2/KDR Antibody Picoband™ (A00901-2)
Hello CJ!
A00901-2 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro
A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis
Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Wu Y, You H, Ma P, Li L, Yuan Y, Li J, Ye X, Liu X, Yao H, Chen R, Lai K, Yang X. Plos One. 2013 May 9;8(5):E62827. Doi: 10.1371/Journal.Pone.0062827. Print 2013. Role Of Transient Receptor Potential Ion Channels And Evoked Levels Of Neuropeptides...
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Customer Q&As
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4 Customer Q&As for Anti-VEGF Receptor 2/KDR Antibody Picoband™
Question
We ordered your anti-VEGF Receptor 2/KDR antibody for Flow Cytometry on lung in a previous project. I am using human, and We are going to use the antibody for ELISA next. We are interested in examining lung as well as umbilical vein in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
I took a look at the website and datasheets of our anti-VEGF Receptor 2/KDR antibody and it seems that A00901-2 has been validated on human in both Flow Cytometry and ELISA. Thus A00901-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-05
Question
My boss were satisfied with the WB result of your anti-VEGF Receptor 2/KDR antibody. However we have been able to see positive staining in lung cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
Based on literature, lung does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2018-06-20
Question
We are currently using anti-VEGF Receptor 2/KDR antibody A00901-2 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
The anti-VEGF Receptor 2/KDR antibody (A00901-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-06
Question
We have been able to see staining in human lung. Any tips? Is anti-VEGF Receptor 2/KDR antibody supposed to stain lung positively?
L. Zhao
Verified customer
Asked: 2013-10-15
Answer
According to literature lung does express KDR. According to Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2013-10-15