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Product Info Summary
| SKU: | PA1989 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-VEGF Receptor 2/KDR Antibody Picoband®
View all VEGFR2/KDR/Flk-1 Antibodies
SKU/Catalog Number
PA1989
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF Receptor 2/KDR Antibody catalog # PA1989. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF Receptor 2/KDR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1989)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1989 is reactive to KDR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
151527 MW
Background of VEGFR2/KDR/Flk-1
KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al. (1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells (Plate et al., 1993). Flk1 is the mouse homolog of KDR (Matthews et al., 1991).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1989 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Cell
IHC: Human Lung Cancer Tissue
IHC-F: Human Placenta Tissue
ICC: HELA Cell
Validation Images & Assay Conditions
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Anti-VEGF Receptor 2 antibody, PA1989, Western blotting
WB: SMMC Cell Lysate
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Anti-VEGF Receptor 2 antibody, PA1989, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-VEGF Receptor 2 antibody, PA1989, ICC
ICC: HELA Cell
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Anti-VEGF Receptor 2 antibody, PA1989, IHC(F)
IHC(F): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For KDR (Source: Uniprot.org, NCBI)
Gene Name
KDR
Full Name
Vascular endothelial growth factor receptor 2
Weight
151527 MW
Superfamily
protein kinase superfamily
Alternative Names
Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD309;KDR;FLK1, VEGFR2; KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDR, check out the KDR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGF Receptor 2/KDR Antibody Picoband® (PA1989)
Hello CJ!
PA1989 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis
Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.
Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.
Wu Y, You H, Ma P, Li L, Yuan Y, Li J, Ye X, Liu X, Yao H, Chen R, Lai K, Yang X. Plos One. 2013 May 9;8(5):E62827. Doi: 10.1371/Journal.Pone.0062827. Print 2013. Role Of Transient Receptor Potential Ion Channels And Evoked Levels Of Neuropeptides...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-VEGF Receptor 2/KDR Antibody Picoband®
Question
I see that the anti-VEGF Receptor 2/KDR antibody PA1989 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-20
Question
you antibody to test anti-VEGF Receptor 2/KDR antibody PA1989 on human umbilical vein for research purposes, then I may be interested in using anti-VEGF Receptor 2/KDR antibody PA1989 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The products we sell, including anti-VEGF Receptor 2/KDR antibody PA1989, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-18
Question
Would anti-VEGF Receptor 2/KDR antibody PA1989 work for ICC with umbilical vein?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
According to the expression profile of umbilical vein, KDR is highly expressed in umbilical vein. So, it is likely that anti-VEGF Receptor 2/KDR antibody PA1989 will work for ICC with umbilical vein.
Boster Scientific Support
Answered: 2019-12-18
Question
I was wanting to use your anti-VEGF Receptor 2/KDR antibody for ICC for human umbilical vein on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical vein identification?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, PA1989 anti-VEGF Receptor 2/KDR antibody has been tested for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical vein in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-25
Question
We are currently using anti-VEGF Receptor 2/KDR antibody PA1989 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The anti-VEGF Receptor 2/KDR antibody (PA1989) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-05
Question
Does anti-VEGF Receptor 2/KDR antibody PA1989 work on goat WB with lung?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Our lab technicians have not tested anti-VEGF Receptor 2/KDR antibody PA1989 on goat. You can run a BLAST between goat and the immunogen sequence of anti-VEGF Receptor 2/KDR antibody PA1989 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
Would PA1989 anti-VEGF Receptor 2/KDR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
It shows on the product datasheet, PA1989 anti-VEGF Receptor 2/KDR antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-31
Question
We purchased anti-VEGF Receptor 2/KDR antibody for ICC on lung a few years ago. I am using human, and We are going to use the antibody for IHC next. My question regards examining lung as well as umbilical vein in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
I looked at the website and datasheets of our anti-VEGF Receptor 2/KDR antibody and I see that PA1989 has been validated on human in both ICC and IHC. Thus PA1989 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-06
Question
Is a blocking peptide available for product anti-VEGF Receptor 2/KDR antibody (PA1989)?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
We do provide the blocking peptide for product anti-VEGF Receptor 2/KDR antibody (PA1989). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-28
Question
I have attached the WB image, lot number and protocol we used for umbilical vein using anti-VEGF Receptor 2/KDR antibody PA1989. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-05
Question
Is this PA1989 anti-VEGF Receptor 2/KDR antibody reactive to the isotypes of KDR?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
The immunogen of PA1989 anti-VEGF Receptor 2/KDR antibody is A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2(454-469aa HHIHWYWQLEEECANE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-15
Question
I am interested in using your anti-VEGF Receptor 2/KDR antibody for endothelial cell differentiation studies. Has this antibody been tested with western blotting on smmc cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
I appreciate your inquiry. This PA1989 anti-VEGF Receptor 2/KDR antibody is tested on smmc cell lysate, human placenta tissue, lung cancer tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-11
Question
Do you have a BSA free version of anti-VEGF Receptor 2/KDR antibody PA1989 available?
S. Krishna
Verified customer
Asked: 2017-04-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF Receptor 2/KDR antibody PA1989 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-04-12
Question
My boss were satisfied with the WB result of your anti-VEGF Receptor 2/KDR antibody. However we have seen positive staining in lung isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?
F. Mitchell
Verified customer
Asked: 2016-12-16
Answer
From literature, lung does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2016-12-16
Question
We have seen staining in human umbilical vein. What should we do? Is anti-VEGF Receptor 2/KDR antibody supposed to stain umbilical vein positively?
C. Moore
Verified customer
Asked: 2016-11-23
Answer
Based on literature umbilical vein does express KDR. Based on Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2016-11-23
Question
Can you help my question with product PA1989, anti-VEGF Receptor 2/KDR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Anderson
Verified customer
Asked: 2015-05-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1989 anti-VEGF Receptor 2/KDR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-05-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein using anti-VEGF Receptor 2/KDR antibody PA1989. Let me know if you need anything else.
Z. Patel
Verified customer
Asked: 2014-04-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-10
