Click image to see more details
Product Info Summary
| SKU: | EK0590 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse VEGFR2/KDR ELISA Kit PicoKine®
View all VEGFR2/KDR/Flk-1 ELISA kits
SKU/Catalog Number
EK0590
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse VEGFR2/KDR ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Kdr in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse VEGFR2/KDR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0590)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A20-E762
Sensitivity
<2 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0590 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0590 is reactive to Kdr in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0590 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR2/KDR/Flk-1
Vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0590-CAP | Anti-Mouse Kdr Pre-coated 96-well strip microplate | 1 |
| EK0590-ST | Mouse Kdr Standard | 2 vials, 10 ng/tube |
| EK0590-DA | Mouse Kdr Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.015 | 0.105 | 0.160 | 0.287 | 0.514 | 0.903 | 1.463 | 2.077 |
Data Example Images
Click image to see more details
Mouse VEGFR2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFR2/KDR/Flk-1, Dilution ratio of 1:4, concentration in serum is 20-40 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse VEGFR2/KDR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 134 | 585 | 1309 | 133 | 543 | 1323 |
| Standard deviation | 6.83 | 36.27 | 60.21 | 9.31 | 39.09 | 63.5 |
| CV (%) | 4.8%% | 6.2% | 4.6% | 7% | 7.2% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFR2/KDR/Flk-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 134 | 118 | 135 | 128 | 128 | 6.75 | 5.2% |
| Sample 2 | 585 | 644 | 654 | 632 | 628 | 26.43 | 4.2% |
| Sample 3 | 1309 | 1087 | 1250 | 1292 | 1234 | 87.82 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For Kdr (Source: Uniprot.Org, NCBI)
Gene Name
Kdr
Full Name
Vascular endothelial growth factor receptor 2
Weight
152517 MW
Superfamily
protein kinase superfamily
Alternative Names
Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase NYK;Protein-tyrosine kinase receptor flk-1;CD309;Kdr;Flk-1, Flk1; KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Kdr, check out the Kdr Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Kdr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse VEGFR2/KDR ELISA Kit PicoKine® (EK0590)
Hello CJ!
EK0590 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epigallocatechin-3-gallate promotes angiogenesis via up-regulation of Nfr2 signaling pathway in a mouse model of ischemic stroke
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Mouse VEGFR2/KDR ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Mouse VEGFR2/KDR ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Mouse VEGFR2/KDR ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Hernandez
Verified customer
Asked: 2020-06-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-23
Question
Q: Can VEGFR2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
H. Zhou
Verified customer
Asked: 2019-09-13
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-09-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Bhatt
Verified customer
Asked: 2018-12-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring VEGFR2 using the EK0590 Mouse VEGFR2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-11-20
Question
Q: how to thaw whole blood sample for VEGFR2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-04-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test VEGFR2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-04-08
Question
Q: can you suggest the dilution ratio of serum samples for detection of VEGFR2 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
J. Kulkarni
Verified customer
Asked: 2014-04-18
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the VEGFR2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-04-18
