Click image to see more details
Product Info Summary
| SKU: | M02632 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-VE-Cadherin Cdh5-Rabbit Monoclonal Antibody
View all VE-Cadherin Antibodies
SKU/Catalog Number
M02632
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VE-Cadherin Cdh5-Rabbit Monoclonal Antibody catalog # M02632. Tested in WB, ICC/IF, IP applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VE-Cadherin Cdh5-Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02632)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IIC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from mouse VE Cadherin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02632 is reactive to Cdh5 in Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
88 kDa
Calculated molecular weight
88 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02632 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:500-1:1000
IP 1:50
Positive Control
WB: Mouse lung
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cdh5 using anti-Cdh5 antibody (M02632).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdh5 antigen affinity purified monoclonal antibody (Catalog # M02632) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdh5 at approximately 88 kDa. The expected band size for Cdh5 is at 88 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Cdh5 (Source: Uniprot.org, NCBI)
Gene Name
Cdh5
Full Name
Cadherin-5
Weight
88 kDa
Alternative Names
Cadherin-5;Vascular endothelial cadherin;VE-cadherin;CD144;Cdh5; CDH5 7B4, CD144 cadherin 5 cadherin-5|7B4 |VE-cadherin|cadherin 5, type 2 (vascular endothelium)|cadherin 5, type 2, VE-cadherin (vascular epithelium)|cd144 |endothelial-specific cadherin|vascular endothelial cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cdh5, check out the Cdh5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdh5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VE-Cadherin Cdh5-Rabbit Monoclonal Antibody (M02632)
Hello CJ!
M02632 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li J,Chen H,Lou J,Bao G,Wu C,Lou Z,Wang X,Ding J,Li Z,Xiao J,Xu H,Gao W,Zhou K.Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.J Cell Physiol.2020 Oct 12.doi:10.1002/jcp.30102.Epub ahead of print.PMID:33044023.
Species: Mouse
M02632 usage in article: APP:WB, SAMPLE:FLAP TISSUE, DILUTION:1:1000
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