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Product Info Summary
| SKU: | A04402-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-VASH1 Antibody
SKU/Catalog Number
A04402-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VASH1 Antibody (Catalog # A04402-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VASH1 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-VASH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04402-2)
Host
Rabbit
Contents
VASH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VASH1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1. The immunogen is located within amino acids 290 - 340 of VASH1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist; this antibody will recognize only the long isoform.
Reactive Species
A04402-2 is reactive to VASH1 in Human, Mouse, Rat
Applications
A04402-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
40957 MW
Background of Vasohibin
VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VASH1 antibody can be used for detection of VASH1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.
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Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For VASH1 (Source: Uniprot.org, NCBI)
Gene Name
VASH1
Full Name
Tubulinyl-Tyr carboxypeptidase 1
Weight
40957 MW
Superfamily
transglutaminase-like superfamily
Alternative Names
KIAA1036; KIAA1036vasohibin-1; VASH; VASH1; vasohibin 1; Vasohibin VASH1 KIAA1036, TTCP 1 vasohibin 1 tubulinyl-Tyr carboxypeptidase 1|tubulin carboxypeptidase 1|tyrosine carboxypeptidase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VASH1, check out the VASH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VASH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VASH1 Antibody (A04402-2)
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