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Product Info Summary
| SKU: | A04402-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Vasohibin VASH1 Antibody
SKU/Catalog Number
A04402-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Vasohibin VASH1 Antibody catalog # A04402-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vasohibin VASH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04402-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human Vasohibin. at AA rangle: 230-310
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04402-1 is reactive to VASH1 in Human, Mouse, Rat
Applications
A04402-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
40957 MW
Background of Vasohibin
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTS alpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using Vasohibin Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For VASH1 (Source: Uniprot.org, NCBI)
Gene Name
VASH1
Full Name
Tubulinyl-Tyr carboxypeptidase 1
Weight
40957 MW
Superfamily
transglutaminase-like superfamily
Alternative Names
KIAA1036; KIAA1036vasohibin-1; VASH; VASH1; vasohibin 1; Vasohibin VASH1 KIAA1036, TTCP 1 vasohibin 1 tubulinyl-Tyr carboxypeptidase 1|tubulin carboxypeptidase 1|tyrosine carboxypeptidase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VASH1, check out the VASH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VASH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vasohibin VASH1 Antibody (A04402-1)
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No publications found for A04402-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Vasohibin VASH1 Antibody
Question
We are currently using anti-Vasohibin antibody A04402-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-Vasohibin antibody (A04402-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
Is this A04402-1 anti-Vasohibin antibody reactive to the isotypes of VASH1?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The immunogen of A04402-1 anti-Vasohibin antibody is Synthesized peptide derived from the Internal region of human Vasohibin. at AA rangle: 230-310. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-17
Question
Does anti-Vasohibin antibody A04402-1 work on canine WB with substantia nigra?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Our lab technicians have not validated anti-Vasohibin antibody A04402-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Vasohibin antibody A04402-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine substantia nigra in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
Will anti-Vasohibin antibody A04402-1 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
According to the expression profile of brain, VASH1 is highly expressed in brain. So, it is likely that anti-Vasohibin antibody A04402-1 will work for WB with brain.
Boster Scientific Support
Answered: 2019-06-20
Question
Do you have a BSA free version of anti-Vasohibin antibody A04402-1 available?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vasohibin antibody A04402-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-Vasohibin antibody A04402-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-21
Question
My question regards to test anti-Vasohibin antibody A04402-1 on mouse brain for research purposes, then I may be interested in using anti-Vasohibin antibody A04402-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
The products we sell, including anti-Vasohibin antibody A04402-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-26
Question
Will A04402-1 anti-Vasohibin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
It shows on the product datasheet, A04402-1 anti-Vasohibin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-20
Question
I was wanting to use your anti-Vasohibin antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
It shows on the product datasheet, A04402-1 anti-Vasohibin antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Vasohibin antibody A04402-1. Let me know if you need anything else.
N. Patel
Verified customer
Asked: 2018-04-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-10
Question
I have a question about product A04402-1, anti-Vasohibin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04402-1 anti-Vasohibin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-17
Question
Is a blocking peptide available for product anti-Vasohibin antibody (A04402-1)?
R. Carter
Verified customer
Asked: 2015-10-28
Answer
We do provide the blocking peptide for product anti-Vasohibin antibody (A04402-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-28
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Vasohibin antibody A04402-1. Please let me know if you require anything else.
C. Li
Verified customer
Asked: 2014-01-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-13
