Anti-HIF3/HIF3A Antibody Picoband®

Product Name

Anti-HIF3/HIF3A Antibody Picoband®

View all HIF-3 alpha Antibodies

SKU/Catalog Number

PA2145

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HIF3/HIF3A Antibody catalog # PA2145. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HIF3/HIF3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2145)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2145 is reactive to HIF3A in Human, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

49 kDa

Calculated molecular weight

72433 MW

Background of HIF-3 alpha

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2145 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human

Positive Control

WB: Rat Brain Tissue

Validation Images & Assay Conditions

Gene/Protein Information For HIF3A (Source: Uniprot.org, NCBI)

Alternative Names

Hypoxia-inducible factor 3-alpha ;HIF-3-alpha ;HIF3-alpha;Basic-helix-loop-helix-PAS protein MOP7;Class E basic helix-loop-helix protein 17;bHLHe17;HIF3-alpha-1;Inhibitory PAS domain protein;IPAS;Member of PAS protein 7;PAS domain-containing protein 7;HIF3A ;BHLHE17, MOP7, PASD7; Hif3a|Ipas, MO, MOP7, N, NEPAS, bHLHe1, bHLHe17|hypoxia inducible factor 3, alpha subunit|hypoxia-inducible factor 3-alpha|HIF-3-alpha|HIF3-alpha|HIF3-alpha-1|basic-helix-loop-helix-PAS protein MOP7|hypoxia inducible factor three alpha|inhibitory PAS domain protein|member of PAS protein 7|neonatal and embryonic PAS protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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