Product Info Summary
SKU: | A00578Y594 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-EphA2/5 (Y594) Antibody
SKU/Catalog Number
A00578Y594
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-EphA2/5 (Y594) Antibody catalog # A00578Y594. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-EphA2/5 (Y594) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00578Y594)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human EphA2/5 around the phosphorylation site of Y594.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00578Y594 is reactive to EPHA2 in Human, Mouse
Applications
A00578Y594 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
108266 MW
Background of EPHA2
Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes By similarity. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.
Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
![a00578y594 anti phospho epha2 5 y594 antibody wb testing 1 a00578y594 anti phospho epha2 5 y594 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00578y594-anti-phospho-epha2-5-y594-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-Phospho-EphA2/5 (Y594) Antibody A00578Y594
Western Blot (WB) analysis of JK cells using Phospho-EphA2/5 (Y594) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EPHA2 (Source: Uniprot.org, NCBI)
Gene Name
EPHA2
Full Name
Ephrin type-A receptor 2
Weight
108266 MW
Superfamily
protein kinase superfamily
Alternative Names
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK EPHA2 ARCC2, CTPA, CTPP1, CTRCT6, ECK EPH receptor A2 ephrin type-A receptor 2|epithelial cell receptor protein tyrosine kinase|soluble EPHA2 variant 1|tyrosine-protein kinase receptor ECK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHA2, check out the EPHA2 Infographic
![EPHA2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-EphA2/5 (Y594) Antibody (A00578Y594)
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