Key=store_id
Key=entity_id, value=47589
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=EK1885
Key=has_options, value=0
Key=required_options, value=0
Key=created_at, value=2018-08-21 11:32:44
Key=updated_at, value=2024-11-13 06:01:48
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Mouse SerpinE2 ELISA Kit PicoKine®
Key=cj_search_index
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Key=is_recurring, value=No
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Key=fb_product, value=
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Key=template, value=elisa-kits
Key=price, value=750.0000
Key=weight, value=1.0000
Key=meta_title, value=Mouse SerpinE2 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Mouse SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.. Sensitivity: 0.1ng/ml.
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Key=thumbnail, value=/e/k/ek1885.png
Key=url_key, value=mouse-serpine2-picokine-trade-elisa-kit-ek1885-boster
Key=url_path, value=mouse-serpine2-picokine-trade-elisa-kit-ek1885-boster
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Key=image_label, value=Mouse SerpinE2 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Mouse SerpinE2 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Mouse SerpinE2 PicoKine ELISA Kit standard curve
Key=msrp_display_actual_price_type, value=
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Key=size, value=96 wells/kit, with removable strips.
Key=gene_full_name, value=serine (or cysteine) peptidase inhibitor, clade E, member 2
Key=uniprot_id, value=Q07235
Key=assay_range, value=0.78 ng/ml - 50 ng/ml
Key=sensitivity, value=<0.1 ng/ml
Key=applications, value=ELISA
Key=reactivity, value=Mouse
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=A polyclonal antibody from goat|a biotinylated detection polyclonal antibody from goat
Key=anticoagulant, value=heparin, EDTA or citrate
Key=assay_procedure_step_1, value=50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml, 0.78 ng/ml
Key=tmb_incubation_time, value=15-25min
Key=predicted_reactivity
Key=source_company, value=Boster EK1885
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Cardiovascular, Cell Adhesion Proteins, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes
Key=rating_value, value=100
Key=review_count, value=4
Key=phospho_site
Key=mp_exclude_sitemap, value=Yes
Key=cj_related_products, value=Plasminogen Activation:EK1886|PA1348|EK0897|PB9345|EZ0897
Key=description, value=Mouse SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Mouse SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=background, value=Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.
Key=gene_name, value=Serpine2
Key=synonyms, value=Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serine protease-inhibitor 4; Serpin E2; Serpine2; Pi7; Pn1; Spi4
Key=sample_type, value=cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.
Key=kit_components, value=50 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	599	2848	10128	604	2990	10197
Standard deviation	33.54	165.18	455.76	36.84	191.36	723.99
CV (%)	5.6%	5.8%	4.5%	6.1%	6.4%	7.1%

Key=application_details, value=Dilution ratio of 1:10000, concentration in serum and plasma is 30-70 ug/ml.
Key=subcellular_localization, value=extracellular space
Key=tissue_specificity, value=Most abundant in seminal vesicles.
Key=protein_function, value=Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
Key=sample_data, value=

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.089	0.125	0.181	0.287	0.449	0.886	1.85	2.043

Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: S20-P397
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	599	657	572	652	620	35.83	5.7%
Sample 2	2848	2654	2960	2710	2793	119.50	4.2%
Sample 3	10128	10127	9926	9025	9801	455.79	4.6%

*number of samples for each test n=16.
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