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Product Info Summary
| SKU: | A05219 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ULK2 Antibody
SKU/Catalog Number
A05219
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ULK2 Antibody catalog # A05219. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ULK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05219)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 930-1000
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05219 is reactive to ULK2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
112694 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05219 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:5000-10000
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Western blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000
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Figure 2. Western blot analysis of ULK2 using Anti-ULK2 Antibody(A05219).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with skim milk for 1.5 hour at RT. The membrane was incubated with rabbit Anti-ULK2 antigen affinity purified polyclonal antibody (A05219) at 1:5000 for 24h at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:3000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK2 at approximately 112 kDa. The expected band size for ULK2 is at 112 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ULK2 (Source: Uniprot.org, NCBI)
Gene Name
ULK2
Full Name
Serine/threonine-protein kinase ULK2
Weight
112694 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase ULK2;2.7.11.1;Unc-51-like kinase 2;ULK2;KIAA0623; ULK2 ATG1B, Unc51.2 unc-51 like autophagy activating kinase 2 serine/threonine-protein kinase ULK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ULK2, check out the ULK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ULK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ULK2 Antibody (A05219)
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No publications found for A05219
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Customer Reviews
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1 Reviews For Anti-ULK2 Antibody
0
Anti-ULK2 Antibody Works Great for Western Blotting
Excellent
Source: Biocompare.com
| Application | Western Blot |
|---|---|
| Sample | HEK293T |
| Primary Incubation | 1:5000 for 24hours at 4degrees celsius. |
| Blocking Agent | Skim milk |
| Secondary Incubation | 1 hour room temperature 1:3000 |
| Detection | ECL |
| Results Summary | We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins. |
"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."
Bence Hajdú
Verified customer
Submitted 2024-07-15
Customer Q&As
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7 Customer Q&As for Anti-ULK2 Antibody
Question
Will anti-ULK2 antibody A05219 work on bovine WB with brain?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Our lab technicians have not tested anti-ULK2 antibody A05219 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ULK2 antibody A05219 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-12
Question
We are currently using anti-ULK2 antibody A05219 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The anti-ULK2 antibody (A05219) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-ULK2 antibody A05219. Let me know if you need anything else.
C. Patel
Verified customer
Asked: 2019-05-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
Is a blocking peptide available for product anti-ULK2 antibody (A05219)?
A. Taylor
Verified customer
Asked: 2018-02-05
Answer
We do provide the blocking peptide for product anti-ULK2 antibody (A05219). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-05
Question
Can you help my question with product A05219, anti-ULK2 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05219 anti-ULK2 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-10
Question
Would A05219 anti-ULK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Johnson
Verified customer
Asked: 2016-01-13
Answer
You can see on the product datasheet, A05219 anti-ULK2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-01-13
Question
Is this A05219 anti-ULK2 antibody reactive to the isotypes of ULK2?
D. Parker
Verified customer
Asked: 2013-06-10
Answer
The immunogen of A05219 anti-ULK2 antibody is Synthetic peptide from AA range: 930-1000. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-06-10
