Anti-ATG14 Antibody

Product Name

Anti-ATG14 Antibody

View all ATG14 Antibodies

SKU/Catalog Number

A03546

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ATG14 Antibody (Catalog # A03546). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ATG14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03546)

Host

Rabbit

Contents

ATG14 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG14 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14. The immunogen is located within amino acids 270 - 320 of ATG14.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms

Reactive Species

A03546 is reactive to ATG14 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

55309 MW

Background of ATG14

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03546 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG14 antibody can be used for detection of ATG14 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ATG14 (Source: Uniprot.org, NCBI)

Alternative Names

ATG14L, BARKOR, KIAA0831, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Barkor ATG14 ATG14L, BARKOR, KIAA0831 autophagy related 14 beclin 1-associated autophagy-related key regulator|ATG14 autophagy related 14 homolog|Beclin 1-Interacting protein|autophagy-related protein 14-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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