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Product Info Summary
| SKU: | A05219-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ULK2 Antibody
SKU/Catalog Number
A05219-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ULK2 Antibody (Catalog # A05219-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
ULK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ULK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05219-1)
Host
Rabbit
Contents
ULK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ULK2 antibody was raised against a 17 amino acid peptide near the center of human ULK2 . The immunogen is located within amino acids 480 - 530 of ULK2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ULK2 are known to exist; this antibody will detect both isoforms. ULK2 antibody is predicted to not cross-react with ULK1.
Reactive Species
A05219-1 is reactive to ULK2 in Human
Applications
A05219-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
112694 MW
Background of ULK2
ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ULK2 Antibody can be used for detection of ULK2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ULK2 in human brain tissue lysate with ULK2 antibody at 1 μg/mL.
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Immunohistochemistry of ULK2 in human brain tissue with ULK2 antibody at 2.5 μg/ml.
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Immunofluorescence of ULK2 in human brain tissue with ULK2 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For ULK2 (Source: Uniprot.org, NCBI)
Gene Name
ULK2
Full Name
Serine/threonine-protein kinase ULK2
Weight
112694 MW
Superfamily
protein kinase superfamily
Alternative Names
ATG1B; EC 2.7.11; EC 2.7.11.1; KIAA0623serine/threonine-protein kinase ULK2; unc-51 (C. elegans)-like kinase 2; Unc51.2; unc-51-like kinase 2 (C. elegans); Unc-51-like kinase 2 ULK2 ATG1B, Unc51.2 unc-51 like autophagy activating kinase 2 serine/threonine-protein kinase ULK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ULK2, check out the ULK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ULK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ULK2 Antibody (A05219-1)
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