Product Info Summary
SKU: | PA1910 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Netrin receptor DCC DCC Antibody Picoband®
SKU/Catalog Number
PA1910
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Netrin receptor DCC DCC Antibody catalog # PA1910. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Netrin receptor DCC DCC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1910)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DCC, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1910 is reactive to DCC in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
158457 MW
Background of DCC
DCC (Deleted IN Colorectal Carcinoma) also known as CRC18 or CRCR1. The DCC gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response. Heterozygous loss-of-function mutations in DCC can result in congenital mirror movements. Alterations in DCC occur frequently in colorectal cancer. Studying a YAC contig containing the entire DCC coding region, they showed that the DCC gene spans approximately 1.4 Mb. Vogelstein (1995) stated that the precise location of the DCC gene was though to be 18q21.3. DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, and they complement genetic evidence for interactions between DCC and netrin homologs in C. DCC protein could be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, colorectal carcinoma and colorectal liver metastases. DCC may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable (for example, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290. Stein et al. (2001) concluded that DCC plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1910 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, U87 Cell, SW620 Cell, COLO320 Cell, 293T Cell, HELA Cell
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-DCC antibody, PA1910, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: HELA Cell Lysate
Click image to see more details
Anti-DCC antibody, PA1910, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For DCC (Source: Uniprot.org, NCBI)
Gene Name
DCC
Full Name
Netrin receptor DCC
Weight
158457 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Netrin receptor DCC;Colorectal cancer suppressor;Immunoglobulin superfamily DCC subclass member 1;Tumor suppressor protein DCC;DCC;IGDCC1; DCC CRC18, CRCR1, HGPPS2, IGDCC1, MRMV1, NTN1R1 DCC netrin 1 receptor netrin receptor DCC|colorectal cancer suppressor|deleted in colorectal carcinoma|immunoglobulin superfamily DCC subclass member 1|putative colorectal tumor suppressor|tumor suppressor protein DCC
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DCC, check out the DCC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DCC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Netrin receptor DCC DCC Antibody Picoband® (PA1910)
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No publications found for PA1910
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Customer Q&As
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1 Customer Q&As for Anti-Netrin receptor DCC DCC Antibody Picoband®
Question
We are currently using anti-DCC antibody PA1910 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The anti-DCC antibody (PA1910) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-31