Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody

Product Name

Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody

View all TSHR Antibodies

SKU/Catalog Number

M00576

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody (Catalog # M00576). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00576)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM222

Isotype

IgG2a, kappa

Immunogen

Recombinant human TSHR, B-Chain protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00576 is reactive to TSHR in Human

Reconstitution

Reconstitute with distilled water.

Calculated molecular weight

86830 MW

Background of TSHR

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Grave's disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00576 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TSHR (Source: Uniprot.org, NCBI)

Alternative Names

Thyrotropin receptor;Thyroid-stimulating hormone receptor;TSH-R;TSHR;LGR3; TSHR CHNG1, LGR3, hTSHR-I thyroid stimulating hormone receptor thyrotropin receptor|TSH receptor|seven transmembrane helix receptor|thyrotropin receptor-I, hTSHR-I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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