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Product Info Summary
| SKU: | A00581-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PTPN22 Antibody Picoband®
View all Lyp/PTPN22 Antibodies
SKU/Catalog Number
A00581-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTPN22 Antibody Picoband® catalog # A00581-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTPN22 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00581-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse PTPN22 recombinant protein (Position: M1-Q309).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00581-1 is reactive to Ptpn22 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
92 kDa
Calculated molecular weight
86830 MW
Background of Lyp/PTPN22
Protein tyrosine phosphatase, non-receptor type 22 (lymphoid), also known as PTPN22, is a protein that in humans is encoded by the PTPN22 gene. This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00581-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat thymus tissue, human SW579 whole cell, human HepG2 whole cell, human placenta tissue, human Hela whole cell, human SW620 whole cell
IHC: mouse spleen tissue, rat lymphaden tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody (Catalog # A00581-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 92KD. The expected band size for PTPN22 is at 92KD.
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Figure 2. IHC analysis of PTPN22 using anti-PTPN22 antibody (A00581-1).
PTPN22 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTPN22 Antibody (A00581-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of PTPN22 using anti-PTPN22 antibody (A00581-1).
PTPN22 was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTPN22 Antibody (A00581-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human SW620 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody (Catalog # A00581-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 92KD. The expected band size for PTPN22 is at 92KD.
Protein Target Info & Infographic
Gene/Protein Information For Ptpn22 (Source: Uniprot.org, NCBI)
Gene Name
Ptpn22
Full Name
Tyrosine-protein phosphatase non-receptor type 22
Weight
86830 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Tyrosine-protein phosphatase non-receptor type 22; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; PEST-domain phosphatase; PEP; Ptpn22; Ptpn8 Ptpn22|70zp, 70zpep, PEP, Pt, Ptpn8|protein tyrosine phosphatase, non-receptor type 22 (lymphoid)|tyrosine-protein phosphatase non-receptor type 22|PEST domain-enriched tyrosine phosphatase|hematopoietic cell protein-tyrosine phosphatase 70Z-PEP|protein tyrosine phosphatase, non-receptor type 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ptpn22, check out the Ptpn22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ptpn22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTPN22 Antibody Picoband® (A00581-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PTPN22 Antibody Picoband®
Question
We are currently using anti-PTPN22 antibody A00581-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-PTPN22 antibody (A00581-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
Does A00581-1 antibody reacts with human PTPN22? Is it specific for mouse PTPN22 only or does it react with other kinases as well?
Verified customer
Asked: 2019-10-11
Answer
Yes, our Anti-PTPN22 Antibody Picoband A00581-1 reacts with human PTPN22. Our lab has not experimentally validated the cross reactivity with other kinases since the sequence homology with other kinase is not very high.
Boster Scientific Support
Answered: 2019-10-15
Question
Is there a BSA free version of anti-PTPN22 antibody A00581-1 available?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PTPN22 antibody A00581-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-28
Question
See attached the WB image, lot number and protocol we used for lymph using anti-PTPN22 antibody A00581-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-01
