Product Info Summary
SKU: | EK7098 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
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Product info
Product Name
Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit
SKU/Catalog Number
EK7098
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Thyroid-Stimulating Hormone (TSH). 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7098)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.10 mIU/L
Assay Range
0.3-12 mIU/L
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7098 is reactive to TSHR in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7098 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Anti-Human Thyroid-Stimulating Hormone (TSH) Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti- Thyroid-Stimulating Hormone (TSH) monoclonal antibody, Polystyrene micro-well plate |
Human Thyroid-Stimulating Hormone (TSH) Standards (S0~S6) | 7 | 1ml | Thyroid-Stimulating Hormone (TSH) (0, 0.3, 0.6, 1.5, 3.0, 6.0, 12.0mIU/L), 0.02M PBS, 2% BSA, 0.1%Proclin-300 |
HRP Conjugated anti-Human Thyroid-Stimulating Hormone (TSH) antibody | 1 | 6ml | HRP Conjugated anti-Human Thyroid-Stimulating Hormone (TSH) antibody, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, 0.01% azophloxine |
Controls | 2 | 1ml | Thyroid-Stimulating Hormone (TSH), 100% natural protein, 0.1%Proclin-300 |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
![ek7098 ek7098](https://www.bosterbio.com/media/catalog/product/e/k/ek7098.png)
Click image to see more details
Human TSH ELISA Kit standard curve
Specific Publications For Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit (EK7098)
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Customer Reviews
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1 Reviews For Human Thyroid Stimulating Hormone (TSH) OneStep ELISA Kit
0
Thyroid Stimulating Hormone ELISA Kit From Boster Bio Works Well
Excellent
![](/media/images/testimonials/EK7098-Madison Schwartz.png)
Source: Biocompare.com
Application | Human TSH in dried blood spots |
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Starting Material | Dried blood spots |
Tips | Use the 0 standard as a diluent, PBS works decently if not. |
Results Summary | The standard curve results consistently gave an excellent curve. No values on the curve or within our patient samples fell outside of the range. |
"The purpose of this experiment was to quantify the level of TSH in a dried blood spot. The application of dried blood spots over whole blood or serum is to cater to remote patients and provide at-home healthcare."
Madison Schwartz
Verified customer
Submitted 2024-07-15
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