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Product Info Summary
| SKU: | A00020-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CTLA4 Antibody Picoband®
SKU/Catalog Number
A00020-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CTLA4 Antibody Picoband® catalog # A00020-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTLA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00020-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CTLA4 recombinant protein (Position: E36-D161). Mouse CTLA4 shares 67.5% amino acid (aa) sequence identity with human CTLA4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00020-1 is reactive to Ctla4 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
24993 MW
Background of CTLA-4
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00020-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: mouse NIH3T3 whole Cell
IHC: mouse spleen tissue, rat lymphaden tissue, rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (A00020-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse NIH3T3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody (Catalog # A00020-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD.
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Figure 2. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1).
CTLA4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1).
CTLA4 was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1).
CTLA4 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ctla4 (Source: Uniprot.org, NCBI)
Gene Name
Ctla4
Full Name
Cytotoxic T-lymphocyte protein 4
Weight
24993 MW
Alternative Names
Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;CTLA-4;CD152;Ctla4;Cd152; CTLA4 ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12 cytotoxic T-lymphocyte associated protein 4 cytotoxic T-lymphocyte protein 4|celiac disease 3|cytotoxic T lymphocyte associated 4 short spliced form|cytotoxic T-lymphocyte-associated serine esterase-4|insulin-dependent diabetes mellitus 12|ligand and transmembrane spliced cytotoxic T lymphocyte associated 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ctla4, check out the Ctla4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ctla4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTLA4 Antibody Picoband® (A00020-1)
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Customer Q&As
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17 Customer Q&As for Anti-CTLA4 Antibody Picoband®
Question
What is the formulation for product A00020-1?
Verified customer
Asked: 2021-02-23
Answer
For the Anti-CTLA4 Antibody Picoband™ (A00020-1), each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2.
Boster Scientific Support
Answered: 2021-02-24
Question
I am interested in using your anti-CTLA4 antibody for negative regulation of regulatory t cell differentiation studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thanks for your inquiry. This A00020-1 anti-CTLA4 antibody is validated on mouse spleen tissue, nih3t3 whole cell lysate, rat lymphaden tissue. It is guaranteed to work for ELISA, IHC-P, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-20
Question
We are currently using anti-CTLA4 antibody A00020-1 for rat tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-CTLA4 antibody (A00020-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
I see that the anti-CTLA4 antibody A00020-1 works with IHC-P, what is the protocol used to produce the result images on the product page?
B. Roberts
Verified customer
Asked: 2019-12-12
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-12
Question
Here is the WB image, lot number and protocol we used for lung using anti-CTLA4 antibody A00020-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
Is there a BSA free version of anti-CTLA4 antibody A00020-1 available?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CTLA4 antibody A00020-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-04
Question
We were satisfied with the WB result of your anti-CTLA4 antibody. However we have been able to see positive staining in lymphocyte cell membrane using this antibody. Is that expected? Could you tell me where is CTLA4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Based on literature, lymphocyte does express CTLA4. Generally CTLA4 expresses in cell membrane. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-CTLA4 antibody for IHC-P for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
It shows on the product datasheet, A00020-1 anti-CTLA4 antibody has been tested for ELISA, IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-06
Question
Would anti-CTLA4 antibody A00020-1 work for IHC-P with lung?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
According to the expression profile of lung, CTLA4 is highly expressed in lung. So, it is likely that anti-CTLA4 antibody A00020-1 will work for IHC-P with lung.
Boster Scientific Support
Answered: 2019-05-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-CTLA4 antibody A00020-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-30
Question
Our lab used your anti-CTLA4 antibody for IHC-P on lung in the past. I am using mouse, and We want to use the antibody for ELISA next. My lab would like examining lung as well as lymph node in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
I looked at the website and datasheets of our anti-CTLA4 antibody and it seems that A00020-1 has been validated on mouse in both IHC-P and ELISA. Thus A00020-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-21
Question
I have a question about product A00020-1, anti-CTLA4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00020-1 anti-CTLA4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-13
Question
We have seen staining in mouse lymph node. What should we do? Is anti-CTLA4 antibody supposed to stain lymph node positively?
K. Wu
Verified customer
Asked: 2017-07-07
Answer
From what I have seen in literature lymph node does express CTLA4. From what I have seen in Uniprot.org, CTLA4 is expressed in lymph node, lung, lymphocyte, among other tissues. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2017-07-07
Question
Is this A00020-1 anti-CTLA4 antibody reactive to the isotypes of CTLA4?
D. Patel
Verified customer
Asked: 2016-12-23
Answer
The immunogen of A00020-1 anti-CTLA4 antibody is E. coli-derived mouse CTLA4 recombinant protein (Position: E36-D161). Mouse CTLA4 shares 67.5% amino acid (aa) sequence identity with human CTLA4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-23
Question
My lab would like to test anti-CTLA4 antibody A00020-1 on rat lung for research purposes, then I may be interested in using anti-CTLA4 antibody A00020-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Mangal
Verified customer
Asked: 2015-08-27
Answer
The products we sell, including anti-CTLA4 antibody A00020-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-27
Question
Would A00020-1 anti-CTLA4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Edwards
Verified customer
Asked: 2014-08-07
Answer
You can see on the product datasheet, A00020-1 anti-CTLA4 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-07
Question
Is a blocking peptide available for product anti-CTLA4 antibody (A00020-1)?
M. Edwards
Verified customer
Asked: 2014-03-18
Answer
We do provide the blocking peptide for product anti-CTLA4 antibody (A00020-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-03-18
