Click image to see more details
Product Info Summary
| SKU: | PA1372-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HSD11B1 Antibody Picoband®
View all 11 beta-HSD1 Antibodies
SKU/Catalog Number
PA1372-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSD11B1 Antibody catalog # PA1372-1. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSD11B1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1372-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1372-1 is reactive to HSD11B1 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
32401 MW
Background of 11 beta-HSD1
11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al. (1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al. (2000) mapped the HSD11B1 gene to 1q32-q41.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1372-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human hepatocellular carcinoma tumor tissue, human hepatocellular carcinoma paracancerous tissue, monkey liver tissue, rat liver tissue, mouse liver tissue
IHC: human liver cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (PA1372-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 3: monkey liver tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # PA1372-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.
Click image to see more details
Figure 2. IHC analysis of HSD11B1 using anti-HSD11B1 antibody (PA1372-1).
HSD11B1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B1 Antibody (PA1372-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HSD11B1 (Source: Uniprot.org, NCBI)
Gene Name
HSD11B1
Full Name
Corticosteroid 11-beta-dehydrogenase isozyme 1
Weight
32401 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
Corticosteroid 11-beta-dehydrogenase isozyme 1;1.1.1.146;11-beta-hydroxysteroid dehydrogenase 1;11-DH;11-beta-HSD1;Short chain dehydrogenase/reductase family 26C member 1;HSD11B1;HSD11, HSD11L, SDR26C1; HSD11B1 11-DH, 11-beta-HSD1, CORTRD2, HDL, HSD11, HSD11B, HSD11L, SDR26C1 hydroxysteroid 11-beta dehydrogenase 1 corticosteroid 11-beta-dehydrogenase isozyme 1|short chain dehydrogenase/reductase family 26C member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD11B1, check out the HSD11B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD11B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSD11B1 Antibody Picoband® (PA1372-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HSD11B1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-HSD11B1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-HSD11B1 Antibody Picoband®
Question
We are currently using anti-HSD11B1 antibody PA1372-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The anti-HSD11B1 antibody (PA1372-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-16
Question
Is a blocking peptide available for product anti-HSD11B1 antibody (PA1372-1)?
M. Miller
Verified customer
Asked: 2018-07-11
Answer
We do provide the blocking peptide for product anti-HSD11B1 antibody (PA1372-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-11
Question
Can you help my question with product PA1372-1, anti-HSD11B1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1372-1 anti-HSD11B1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-30
Question
See attached the WB image, lot number and protocol we used for brain using anti-HSD11B1 antibody PA1372-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-04
Question
We are interested in to test anti-HSD11B1 antibody PA1372-1 on rat brain for research purposes, then I may be interested in using anti-HSD11B1 antibody PA1372-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Jha
Verified customer
Asked: 2017-01-25
Answer
The products we sell, including anti-HSD11B1 antibody PA1372-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-25
Question
Is this PA1372-1 anti-HSD11B1 antibody reactive to the isotypes of HSD11B1?
K. Williams
Verified customer
Asked: 2016-03-29
Answer
The immunogen of PA1372-1 anti-HSD11B1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1(16-33aa MAYYYYSANEEFRPEMLQ), different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-03-29
Question
I was wanting to use your anti-HSD11B1 antibody for IHC for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
J. Anderson
Verified customer
Asked: 2014-06-02
Answer
You can see on the product datasheet, PA1372-1 anti-HSD11B1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-02
