Anti-TRIM5 gamma Antibody

Product Name

Anti-TRIM5 gamma Antibody

View all TRIM5 Antibodies

SKU/Catalog Number

A01359

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRIM5 gamma Antibody (Catalog # A01359). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

TRIM5 gamma antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TRIM5 gamma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01359)

Host

Rabbit

Contents

TRIM5 gamma Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TRIM5 gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of human TRIM5 gamma. The immunogen is located within the last 50 amino acids of TRIM5 gamma.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Four alternatively spliced transcript variants have been observed.

Reactive Species

A01359 is reactive to TRIM5 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

56338 MW

Background of TRIM5

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5δ isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01359 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 2 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TRIM5 (Source: Uniprot.org, NCBI)

Alternative Names

RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88 TRIM5 RNF88alpha, TRIM5 tripartite motif containing 5 tripartite motif-containing protein 5|RING-type E3 ubiquitin transferase TRIM5|ring finger protein 88|tripartite motif containing 5 transcript variant iota|tripartite motif containing 5 transcript variant kappa|tripartite motif protein TRIM5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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