Click image to see more details
Product Info Summary
| SKU: | A00061 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CCR5 Antibody Picoband®
SKU/Catalog Number
A00061
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR5 Antibody Picoband® catalog # A00061. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00061)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CCR5, which shares 88% amino acid (aa) sequence identity with both mouse and rat CCR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00061 is reactive to CCR5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
25283 MW
Background of CCR5
Human CC chemokine receptor 5 (CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00061 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human Jurkat cell, human MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CCR5 using anti-CCR5 antibody (A00061).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat cell lysates,
Lane 2: human MCF-7 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR5 antigen affinity purified polyclonal antibody (Catalog # A00061) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR5 at approximately 40KD. The expected band size for CCR5 is at 40KD.
Protein Target Info & Infographic
Gene/Protein Information For CCR5 (Source: Uniprot.org, NCBI)
Gene Name
CCR5
Full Name
C-C chemokine receptor type 5
Weight
25283 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CHEMR13; HIV-1 fusion coreceptor; CD195; CCR5; CMKBR5 CCR5 CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22 C-C motif chemokine receptor 5 C-C chemokine receptor type 5|C-C motif chemokine receptor 5 A159A|HIV-1 fusion coreceptor|chemokine (C-C motif) receptor 5|chemokine receptor CCR5|chemokine recptor CCR5 Delta32|chemr13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR5, check out the CCR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR5 Antibody Picoband® (A00061)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CCR5 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CCR5 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-CCR5 Antibody Picoband®
Question
Is this A00061 anti-CCR5 antibody reactive to the isotypes of CCR5?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The immunogen of A00061 anti-CCR5 antibody is A synthetic peptide corresponding to a sequence of human CCR5 (YSGILKTLLRCRNEKKRHRAVRLIFT). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-16
Question
Our lab want to know about using your anti-CCR5 antibody for fusion of virus membrane with host plasma membrane studies. Has this antibody been tested with western blotting on human jurkat cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
We appreciate your inquiry. This A00061 anti-CCR5 antibody is validated on human jurkat cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-CCR5 antibody A00061. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
Is there a BSA free version of anti-CCR5 antibody A00061 available?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CCR5 antibody A00061 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-23
Question
I have attached the WB image, lot number and protocol we used for macrophage using anti-CCR5 antibody A00061. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Does anti-CCR5 antibody A00061 work for WB with macrophage?
A. Kulkarni
Verified customer
Asked: 2019-06-25
Answer
According to the expression profile of macrophage, CCR5 is highly expressed in macrophage. So, it is likely that anti-CCR5 antibody A00061 will work for WB with macrophage.
Boster Scientific Support
Answered: 2019-06-25
Question
Would A00061 anti-CCR5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
You can see on the product datasheet, A00061 anti-CCR5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-24
Question
Is a blocking peptide available for product anti-CCR5 antibody (A00061)?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
We do provide the blocking peptide for product anti-CCR5 antibody (A00061). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-30
Question
My question regards to test anti-CCR5 antibody A00061 on mouse macrophage for research purposes, then I may be interested in using anti-CCR5 antibody A00061 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
The products we sell, including anti-CCR5 antibody A00061, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-06
Question
We have observed staining in mouse macrophage. Do you have any suggestions? Is anti-CCR5 antibody supposed to stain macrophage positively?
B. Collins
Verified customer
Asked: 2017-07-06
Answer
From literature macrophage does express CCR5. From Uniprot.org, CCR5 is expressed in visceral pleura, macrophage, among other tissues. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314
Boster Scientific Support
Answered: 2017-07-06
Question
We are currently using anti-CCR5 antibody A00061 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
M. Huang
Verified customer
Asked: 2017-05-03
Answer
The anti-CCR5 antibody (A00061) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-03
Question
My team were satisfied with the WB result of your anti-CCR5 antibody. However we have seen positive staining in visceral pleura cell membrane using this antibody. Is that expected? Could you tell me where is CCR5 supposed to be expressed?
G. Rodriguez
Verified customer
Asked: 2016-11-15
Answer
Based on literature, visceral pleura does express CCR5. Generally CCR5 expresses in cell membrane. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314
Boster Scientific Support
Answered: 2016-11-15
Question
I see that the anti-CCR5 antibody A00061 works with WB, what is the protocol used to produce the result images on the product page?
B. Bhatt
Verified customer
Asked: 2016-08-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-12
Question
I was wanting to use your anti-CCR5 antibody for WB for mouse macrophage on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse macrophage identification?
E. Rodriguez
Verified customer
Asked: 2015-09-10
Answer
You can see on the product datasheet, A00061 anti-CCR5 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-10
Question
My question regarding product A00061, anti-CCR5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Evans
Verified customer
Asked: 2013-01-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00061 anti-CCR5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-03
