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Product Info Summary
| SKU: | PA1016-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband®
SKU/Catalog Number
PA1016-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C-C chemokine receptor type 5 CCR5 Antibody catalog # PA1016-2. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1016-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CCR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1016-2 is reactive to Ccr5 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
40785 MW
Background of CCR5
Human CC chemokine receptor 5 (CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1016-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Lung Tissue, Mouse Intestine Tissue, Mouse Kidney Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-CCR5 antibody, PA1016-2, Western blotting
Lane 1: Mouse Lung Tissue Lysate
Lane 2: Mouse Intestine Tissue Lysate
Lane 3: Mouse Kidney Tissue Lysate
Protein Target Info & Infographic
Gene/Protein Information For Ccr5 (Source: Uniprot.org, NCBI)
Gene Name
Ccr5
Full Name
C-C chemokine receptor type 5
Weight
40785 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;MIP-1 alpha receptor;CD195;Ccr5;Cmkbr5; CCR5 CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22 C-C motif chemokine receptor 5 C-C chemokine receptor type 5|C-C motif chemokine receptor 5 A159A|HIV-1 fusion coreceptor|chemokine (C-C motif) receptor 5|chemokine receptor CCR5|chemokine recptor CCR5 Delta32|chemr13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccr5, check out the Ccr5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccr5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband® (PA1016-2)
Hello CJ!
PA1016-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Study on CCR5 analogs and affinity peptides
Li G, Xu H, Zhu S, Xu W, Qin S, Liu S, Tu G, Peng H, Qiu S, Yu S, Zhu Q, Fan B, Zheng C, Li G, Liang S. Brain Res Bull. 2013 Jan;90:79-87. Doi: 10.1016/J.Brainresbull.2012.10.002. Epub 2012 Oct 9. Effects Of Neferine On Ccl5 And Ccr5 Expression In...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband®
Question
Is there a BSA free version of anti-CCR5 antibody PA1016-2 available?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CCR5 antibody PA1016-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-CCR5 antibody PA1016-2 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The anti-CCR5 antibody (PA1016-2) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-16
Question
Would PA1016-2 anti-CCR5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
It shows on the product datasheet, PA1016-2 anti-CCR5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-21
Question
Would anti-CCR5 antibody PA1016-2 work on goat WB with visceral pleura?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Our lab technicians have not tested anti-CCR5 antibody PA1016-2 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CCR5 antibody PA1016-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat visceral pleura in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
Will anti-CCR5 antibody PA1016-2 work for WB with macrophage?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
According to the expression profile of macrophage, CCR5 is highly expressed in macrophage. So, it is likely that anti-CCR5 antibody PA1016-2 will work for WB with macrophage.
Boster Scientific Support
Answered: 2019-11-25
Question
Is a blocking peptide available for product anti-CCR5 antibody (PA1016-2)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-CCR5 antibody (PA1016-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
Our lab were happy with the WB result of your anti-CCR5 antibody. However we have seen positive staining in visceral pleura cell membrane using this antibody. Is that expected? Could you tell me where is CCR5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
According to literature, visceral pleura does express CCR5. Generally CCR5 expresses in cell membrane. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314
Boster Scientific Support
Answered: 2019-08-07
Question
I have attached the WB image, lot number and protocol we used for macrophage using anti-CCR5 antibody PA1016-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-15
Question
My lab would like using your anti-CCR5 antibody for r-mmu-418594; g alpha (i) signalling events studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
We appreciate your inquiry. This PA1016-2 anti-CCR5 antibody is tested on mouse lung tissue, tissue lysate, intestine tissue, kidney tissue. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-22
Question
I see that the anti-CCR5 antibody PA1016-2 works with WB, what is the protocol used to produce the result images on the product page?
G. Bhatt
Verified customer
Asked: 2018-01-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for macrophage using anti-CCR5 antibody PA1016-2. Let me know if you need anything else.
T. Miller
Verified customer
Asked: 2017-02-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-06
Question
Is this PA1016-2 anti-CCR5 antibody reactive to the isotypes of CCR5?
A. Roberts
Verified customer
Asked: 2016-10-26
Answer
The immunogen of PA1016-2 anti-CCR5 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse CCR5(181-199aa SPHFPHTQYHFWKSFQTLK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-10-26
Question
We have seen staining in mouse macrophage. Do you have any suggestions? Is anti-CCR5 antibody supposed to stain macrophage positively?
A. Patel
Verified customer
Asked: 2016-08-25
Answer
Based on literature macrophage does express CCR5. Based on Uniprot.org, CCR5 is expressed in visceral pleura, macrophage, among other tissues. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314
Boster Scientific Support
Answered: 2016-08-25
Question
I was wanting to use to test anti-CCR5 antibody PA1016-2 on mouse macrophage for research purposes, then I may be interested in using anti-CCR5 antibody PA1016-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Evans
Verified customer
Asked: 2014-06-23
Answer
The products we sell, including anti-CCR5 antibody PA1016-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-23
Question
I was wanting to use your anti-CCR5 antibody for WB for mouse macrophage on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse macrophage identification?
L. Evans
Verified customer
Asked: 2014-04-30
Answer
As indicated on the product datasheet, PA1016-2 anti-CCR5 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse macrophage in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-30
Question
Can you help my question with product PA1016-2, anti-CCR5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Gonzalez
Verified customer
Asked: 2013-09-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1016-2 anti-CCR5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-09-11
