CRTC1 Antibodies

CREB-regulated transcription coactivator 1 (CRTC1)

Transcriptional coactivator for CREB1 which activates transcription via both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being busy when dephosphorylated and acts independently of CREB1'Ser-133' phosphorylation.

Regulates the expression of particular CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells.

Gene Information

Human
Gene Name:	CRTC1
Uniprot:	Q6UUV9
Entrez:	23373

Family

Belongs to:
TORC family

Alternative Names

CREB regulated transcription coactivator 1; CRTC1; FLJ14027WAMTP1; KIAA0616Transducer of regulated cAMP response element-binding protein 1; MECT1; MECT1CREB-regulated transcription coactivator 1; mucoepidermoid carcinoma translocated 1; Mucoepidermoid carcinoma translocated protein 1; TORC1; TORC1TORC-1; Transducer of CREB protein 1; transducer of regulated cAMP response element-binding protein (CREB) 1; WAMTP1

Molecular Weight

Mass (kDA):
67.3 kDA

Genomic Context

Human
Location:	19p13.11
Sequence:	19; NC_000019.10 (18683621..18782333)

Tissue Specificity

Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.

Subcellular Localizations

Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Mucoepidermoid Carcinoma
• Tuberous Sclerosis
• Sclerosis
• Diabetes Mellitus
• Cell Transformation, Neoplastic
• Hypertrophy
• Salivary Gland Neoplasms
• Insulin Resistance
• Salivary Gland Diseases
• Malignant Paraganglionic Neoplasm

• Obesity
• Lung Neoplasms
• Neoplasm Metastasis
• Nervousness
• Parotid Gland Warthin Tumor
• Leukemia
• Kidney Diseases

Interacting Proteins

• CREB1

Pathways

• Cell Growth
• Translation
• Autophagy
• Cell Proliferation
• Cell Differentiation
• Aging
• Long-term Memory
• Energy Homeostasis
• Pathogenesis
• Transport
• Localization
• Secretion
• Myelination

• Regeneration
• Cell Cycle
• Synaptic Transmission
• Senescence
• Cell Death
• Cell Migration
• Gluconeogenesis

PTMs

• Phosphorylation
• Dephosphorylation

• Ubiquitination
• Deacetylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CRTC1

Best Uses Of The CRTC1 Marker From Boster Bio

When looking for new ways to test CRTC1 in cells, it is vital to select the right gene therapy. Boster Bio can provide this antibody. Boster Bio offers a broad range of CRTC1-specific reagents to satisfy your requirements. This antibody is particularly useful in investigating human cancers as it identifies the most effective treatment options for various kinds of tumors.

CRTC1

The CRTC1 marker is a crucial biomarker of neuronal plasticity. When stimulated by different stimuli, it undergoes an activity-regulated translocations between the synapse, and the nucleus. Additionally, it displays large changes in its MW that are influenced by activity. This biomarker can be utilized for research and therapy. Let's explore some of the most popular uses of the CRTC1 gene.

The CRTC1 component of the M-M2 oncoprotein is involved in cell proliferation through AP-1 and CREB. Microarray analysis has revealed that the MAML2–CRTC1 interaction is a powerful target for the research and development of new treatments or drugs. These discoveries suggest that the CRTC1MAML2 fusion protein could be a significant therapeutic target in the human MEC.

CRTCs are the primary regulators of the AP-1-dependent cellular proliferation. They are thought to regulate a range of biological events, and their inadequacy in regulation could be responsible for a wide range of disease states. The CRTC1–MAML2 connection promotes transcription via AP-1. However the exact mechanism behind this is not understood. However, it is likely to contribute to cell proliferation by activating transcription via AP-1.

Moreover the gene is linked with human eating behaviour and metabolic factors. It is possible that this gene has an important role in the adipose biochemistry. Furthermore it is possible that the CRTC1 gene may be related to other genes and pathways in human physiology. This includes epigenetics, which is the control mechanism. The CRTC1 gene plays a role in regulating adipogenesis , and could be an important candidate gene for metabolic dysregulation. Therefore, it is important to know the epigenetic regulation of CRTC1 in order to determine the role this gene plays in human obesity.

MEC (mucoepidermoid carcinoma) is the most prevalent malignancy of the salivary glands. It has poor clinical outcomes. Patients with metastatic CRTC1 who have poor outcomes also result of its unresectable nature. Furthermore, tumors that have chromosomal translocation t(11-19) contain MAML2 CRTC1 that plays a significant role in the growth and maintenance of human MEC cells.

Furthermore, CRTC1 and MAML2 fusion oncoproteins interact to the transcription factor CREB, which is responsible for their oncogenic effects. Its functional analysis will aid in understanding the mechanism by which CRTC1-MAML2 interacts with other transcription factors, and also contributes to MEC pathogenesis. This research will provide vital information that will be helpful to further investigate the functional aspects of MEC.

CRTC3 p-CR

If you're searching for the top CRTC1 antibodies, there's various options available. You can find a full set of antibodies from Boster Bio by following the links below. These optimization techniques will help make your research more successful. In addition to these suggestions, you could also consult the Boster Bio user's guide.

This antibody recognizes CRTC3's S391's phosphorylation site. The MAPKs and CDKs are the key players in phosphorylation. It was discovered through Western blot analysis of IPs from CRTC3-FLAG cells. The molecular weight of the antibody is 73 millimeters. The antibody is compatible with both human and mouse cells.

The CRTC2/3 proteins is required for the expression of CCL1 and CXCL2. Both proteins are crucial for the regulation of insulin signaling and glucose metabolism. However their expression is reduced in dAKO Adipocytes. It is therefore vital to understand the role of the CRTC1 and CRTC2/3 genes in expression. To accomplish this scientists have developed antibodies that target CRTC2/3.

The brain and adipose tissues are home to high levels of the CRTC2/3 protein. Double- or triple-knockout mice of the CRTC2/3 genes lower the expression of the protein. To study the role of CRTC2/3 in adiponectin mice, floxed alleles of CRTC2/3 gene were crossed with Adipoq Cre transgenic mice. The CRTC2/3 double knockout mice display a reduction in CRTC2/3 expression of 70 percent.

Mice knocked out of CRTC2

Utilizing Boster Bio CRTC2 knockout mouse lines, researchers can conduct research on the physiological effects of dietary fat on health. These mice have improved glucose and insulin tolerance, and reduced fat mass. The energy metabolism of the dAKO mice has been assessed by indirect calorimetry. The mice consume more oxygen at higher rates and higher energy expenses than their WT littermates. They also show comparable levels of physical activity.

CRTC2 and CRTC3 regulate expression of adipocyte-derived cell cytokines. They are involved in the insulin signaling process and glucose metabolism within adipose tissues. Their role in regulating the metabolism of glucose is not yet clear. Thus, it is necessary to identify other molecular mechanisms by which CRTC2 and CRTC3 may function. In the absence of CRTC2 and CRTC3, insulin levels as well as glucose levels can rise and fall within the body.

The CRTC2 and NFKB p65 proteins co-bind in adipocytes. These two transcription factors trigger adipocytes through a signaling pathway. TNFa stimulates nuclear translocation of the CRTC2 as well as NF-kB-p65. It also increases the presence of H3AcK27 and p65 co-receptors in 3T3-L1 adipocytes.

The overexpression of SIK2 within adipose tissues increases the resistance to insulin. It also promotes the secretion of pro-inflammatory cytokines. SIK2 and CRTCs work in tandem to increase insulin resistance by inducing adipocyte-derived chemicals CXCL1/2. These proteins are important in the development and maintenance of type II diabetes and obesity.

Boster Bio CRTC2 knockout mouse lines also demonstrate the effects of TNFa on the process of adipogenesis. TNFa also inhibits CRTC nuclear translocation within 3T3-L1 Adipocytes. TNFa also inhibits NFkB activation. Furthermore, it also inhibits CRTC nuclear translocation in Adipocytes.