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Product Info Summary
| SKU: | A04408-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-TNFSF18 Antibody Picoband®
View all GITR Ligand/TNFSF18 Antibodies
SKU/Catalog Number
A04408-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNFSF18 Antibody Picoband® catalog # A04408-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNFSF18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04408-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TNFSF18 recombinant protein (Position: F70-S199).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04408-1 is reactive to TNFSF18 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
22.724kDa
Background of GITR Ligand/TNFSF18
Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04408-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
IHC: human colon cancer tissue, human lung cancer tissue, human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TNFSF18 using anti-TNFSF18 antibody (A04408-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human TNFSF18 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF18 antigen affinity purified polyclonal antibody (Catalog # A04408-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFSF18 at approximately 15KD. The expected band size for TNFSF18 is at 15KD.
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Figure 2. IHC analysis of TNFSF18 using anti-TNFSF18 antibody (A04408-1).
TNFSF18 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNFSF18 Antibody (A04408-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of TNFSF18 using anti-TNFSF18 antibody (A04408-1).
TNFSF18 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNFSF18 Antibody (A04408-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TNFSF18 using anti-TNFSF18 antibody (A04408-1).
TNFSF18 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TNFSF18 Antibody (A04408-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF18 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF18
Full Name
Tumor necrosis factor ligand superfamily member 18
Weight
22.724kDa
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 18; Activation-inducible TNF-related ligand; AITRL; Glucocorticoid-induced TNF-related ligand; hGITRL; TNFSF18; AITRL, GITRL, TL6; UNQ149/PRO175; TNFSF18 AITRL, GITRL, TL6, TNLG2A, hGITRL TNF superfamily member 18 tumor necrosis factor ligand superfamily member 18|AITR ligand|GITR ligand|activation-inducible TNF-related ligand|glucocorticoid-induced TNF-related ligand|glucocorticoid-induced TNFR-related protein ligand|tumor necrosis factor (ligand) superfamily, member 18|tumor necrosis factor ligand 2A|tumor necrosis factor superfamily member 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF18, check out the TNFSF18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNFSF18 Antibody Picoband® (A04408-1)
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Customer Q&As
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1 Customer Q&As for Anti-TNFSF18 Antibody Picoband®
Question
We are currently using anti-TNFSF18 antibody A04408-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Z. Collins
Verified customer
Asked: 2016-09-12
Answer
The anti-TNFSF18 antibody (A04408-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-12
