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Product Info Summary
| SKU: | EK1359 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IL-24/Interleukin-24 ELISA Kit PicoKine®
SKU/Catalog Number
EK1359
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-24 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il24 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-24/Interleukin-24 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1359)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q27-L181
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1359 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1359 is reactive to Il24 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1359 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-24
Interleukin 24 (IL-24) is a cytokine belonging to the IL-10 family of cytokines that signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as Melanoma differentiation-associated 7 (mda-7) due to its discovery as a tumour suppressing protein. By radiation hybrid analysis, the MDA7 gene is mapped to 1q32.2-q41. IL-24 appears to control in cell survival and proliferation by inducing rapid activation of particular transcription factors called Stat-1 and Stat-3. Mouse IL24 was expressed as an approximately 35-kD protein that was converted to a 23-kD protein by deglycosylation. The IL24 protein shares 24 to 33% homology with other members of the IL10 family of cytokines, with highest homology with IL20. This cytokine is predominantly released by activated monocytes, macrophages and T helper 2 (Th2) cells and acts on non-haematopoietic tissues such as skin, lung and reproductive tissues. IL-24 performs important roles in wound healing, psoriasis and cancer.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1359-CAP | Anti-Mouse Il24 Pre-coated 96-well strip microplate | 1 |
| EK1359-ST | Mouse Il24 Standard | 2 vials, 10 ng/tube |
| EK1359-DA | Mouse Il24 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.008 | 0.036 | 0.118 | 0.224 | 0.398 | 0.717 | 1.241 | 1.829 |
Data Example Images
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Mouse IL-24 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-24/Interleukin-24 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 142 | 555 | 1654 | 131 | 526 | 1652 |
| Standard deviation | 8.94 | 25.53 | 77.73 | 5.7%%5 | 29.98 | 90.86 |
| CV (%) | 5.7%% | 4.6% | 4.7% | 5.7%% | 5.7% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-24 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 142 | 155 | 146 | 133 | 144 | 7.9 | 5.4% |
| Sample 2 | 555 | 583 | 584 | 618 | 585 | 22.32 | 3.8% |
| Sample 3 | 1654 | 1616 | 1741 | 1741 | 1688 | 54.67 | 3.2% |
Protein Target Info & Infographic
Gene/Protein Information For Il24 (Source: Uniprot.Org, NCBI)
Gene Name
Il24
Full Name
Interleukin-24
Weight
23.825kDa
Superfamily
IL-10 family
Alternative Names
Interleukin-24; IL-24; Melanoma differentiation-associated gene 7 protein; MDA-7; Suppression of tumorigenicity 16 protein; IL24; MDA7; ST16 IL24 C49A, FISP, IL10B, MDA7, MOB5, ST16 interleukin 24 interleukin-24|IL-4-induced secreted protein|melanocyte-associated Mda-7|melanoma differentiation-associated gene 7 protein|suppression of tumorigenicity 16 (melanoma differentiation)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il24, check out the Il24 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il24: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-24/Interleukin-24 ELISA Kit PicoKine® (EK1359)
Hello CJ!
EK1359 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Novel optimized drug delivery systems for enhancing spinal cord injury repair in rats
Polysaccharide from natural Cordyceps sinensis ameliorated intestinal injury and enhanced antioxidant activity in immunosuppressed mice
Khoshroo,M.,Yazdanpanah,M.,Yasrebi,S.(2021).Serum Interleukin-24 Levels in Gastric and Breast Cancers and Non-cancerous Inflammations.Middle East Journal of Cancer,12(2),183-189.doi:10.30476/mejc.2020.82945.1122
Species: Human
EK1359 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Zhao Y, Li Z, Sheng W, Miao J, Yang J. Oncol Rep. 2012 Oct;28(4):1315-24. Doi: 10.3892/Or.2012.1930. Epub 2012 Jul 24. Adenovirus-Mediated Ing4/Il-24 Double Tumor Suppressor Gene Co-Transfer Enhances Antitumor Activity In Human Breast ...
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Customer Q&As
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4 Customer Q&As for Mouse IL-24/Interleukin-24 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for IL-24 ELISA after freezing?
H. Walker
Verified customer
Asked: 2020-06-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-24 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-06-24
Question
Q: What is the optimal O.D. value for IL-24 ELISA kit? I used your IL-24 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-24 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-24 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-24 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: if the enzyme conjugated IL-24 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-24 antigen?
Verified Customer
Verified customer
Asked: 2018-10-20
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-10-20
Question
Q: is it okay to use citrate plasma as samples in Mouse IL-24 Picokine® ELISA Kit (Catalog # EK1359)?
Verified Customer
Verified customer
Asked: 2018-09-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-24 causing disruption of its protein structure. IL-24 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-24 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-09-29
