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Product Info Summary
| SKU: | EK2125 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®
View all SPARC-like 1/SPARCL1 ELISA kits
SKU/Catalog Number
EK2125
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SPARCL1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse SPARCL1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2125)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: I17-F650
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2125 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2125 is reactive to Sparcl1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2125 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SPARC-like 1/SPARCL1
SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. It is mapped to 5 E5; 5 50.55 cM. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2125-CAP | Anti-Mouse Sparcl1 Pre-coated 96-well strip microplate | 1 |
| EK2125-ST | Mouse Sparcl1 Standard | 2 vials, 20 ng/tube |
| EK2125-DA | Mouse Sparcl1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.010 | 0.072 | 0.139 | 0.253 | 0.481 | 0.842 | 1.437 | 2.327 |
Data Example Images
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Mouse SPARCL1 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 467 | 1310 | 7832 | 529 | 2102 | 8406 |
| Standard deviation | 19.15 | 56.33 | 360.27 | 30.68 | 145.04 | 605.23 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.8% | 6.9% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SPARC-like 1/SPARCL1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 467 | 427 | 448 | 472 | 453 | 17.72 | 3.9% |
| Sample 2 | 1310 | 1396 | 1545 | 1357 | 1402 | 87.99 | 6.2% |
| Sample 3 | 7832 | 7176 | 7216 | 8274 | 7624 | 456.32 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For Sparcl1 (Source: Uniprot.Org, NCBI)
Gene Name
Sparcl1
Full Name
SPARC-like protein 1
Weight
75.208kDa
Superfamily
SPARC family
Alternative Names
SPARC-like protein 1; Extracellular matrix protein 2; Matrix glycoprotein Sc1; Sparcl1; Ecm2; Sc1 SPARCL1 MAST 9, MAST9, PIG33, SC1 SPARC like 1 SPARC-like protein 1|SPARC-like 1 (hevin)|high endothelial venule protein|proliferation-inducing protein 33
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Sparcl1, check out the Sparcl1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Sparcl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine® (EK2125)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Clark
Verified customer
Asked: 2020-06-07
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-06-07
Question
Q: is it okay to use EDTA plasma as samples in Mouse SPARC-like 1 Picokine® ELISA Kit (Catalog # EK2125)?
L. Collins
Verified customer
Asked: 2019-11-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of SPARC-like 1 causing degradation of its protein structure. SPARC-like 1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the SPARC-like 1 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-17
Question
Q: can you suggest the dilution ratio of serum samples for detection of SPARC-like 1 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SPARC-like 1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-10-19
Question
Q: What is the optimal O.D. value for SPARC-like 1 ELISA kit? I performed your SPARC-like 1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SPARC-like 1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SPARC-like 1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of SPARC-like 1 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-08-15
Question
Q: how can I thaw whole blood sample for SPARC-like 1 ELISA after freezing?
M. Lopez
Verified customer
Asked: 2018-05-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SPARC-like 1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-05-30
