Anti-ASPP2 TP53BP2 Antibody

Product Name

Anti-ASPP2 TP53BP2 Antibody

View all 53BP2 Antibodies

SKU/Catalog Number

A02609

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ASPP2 TP53BP2 Antibody (Catalog # A02609). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-ASPP2 TP53BP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02609)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02609 is reactive to TP53BP2 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

125616 MW

Background of 53BP2

ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21.  Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02609 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,500 - 1:10,000
IHC: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Gene/Protein Information For TP53BP2 (Source: Uniprot.org, NCBI)

Alternative Names

ASPP2, ASPP-2, ASPP 2, Apoptosis stimulating of p53 protein 2, Bbp, Bcl2 binding protein, NY REN 51 antigen, p53 binding protein 2, Tumor suppressor p53-binding protein 2, TP53BP2 TP53BP2 53BP2, ASPP2, BBP, P53BP2, PPP1R13A tumor protein p53 binding protein 2 apoptosis-stimulating of p53 protein 2|BCL2-binding protein|apoptosis-stimulating protein of p53, 2|renal carcinoma NY-REN-51|tumor suppressor p53-binding protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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