Product Info Summary
SKU: | A30508 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-TM16A ANO1 Antibody
View all DOG1/TMEM16A Antibodies
SKU/Catalog Number
A30508
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TM16A ANO1 Antibody (Catalog# A30508). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, .
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TM16A ANO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30508)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TM16A. Immunogen sequence location: 921-970
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30508 is reactive to ANO1 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
114078 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30508 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of HeLa cells, using TM16A Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ANO1 (Source: Uniprot.org, NCBI)
Gene Name
ANO1
Full Name
Anoctamin-1
Weight
114078 MW
Superfamily
anoctamin family
Alternative Names
ANO1;DOG1;ORAOV2;TAOS2;TMEM16A;Anoctamin-1;Discovered on gastrointestinal stromal tumors protein 1;Oral cancer overexpressed protein 2;Transmembrane protein 16A;Tumor-amplified and overexpressed sequence 2 ANO1 DOG1, ORAOV2, TAOS2, TMEM16A anoctamin 1 anoctamin-1|Ca2+-activated Cl- channel|anoctamin 1, calcium activated chloride channel|calcium activated chloride channel|discovered on gastrointestinal stromal tumors protein 1|oral cancer overexpressed 2|transmembrane protein 16A (eight membrane-spanning domains)|tumor-amplified and overexpressed sequence 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANO1, check out the ANO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TM16A ANO1 Antibody (A30508)
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No publications found for A30508
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Customer Q&As
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4 Customer Q&As for Anti-TM16A ANO1 Antibody
Question
Would anti-TM16A antibody A30508 work for IF with thoracic aorta?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
According to the expression profile of thoracic aorta, ANO1 is highly expressed in thoracic aorta. So, it is likely that anti-TM16A antibody A30508 will work for IF with thoracic aorta.
Boster Scientific Support
Answered: 2020-02-13
Question
Would A30508 anti-TM16A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
It shows on the product datasheet, A30508 anti-TM16A antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-27
Question
Is a blocking peptide available for product anti-TM16A antibody (A30508)?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
We do provide the blocking peptide for product anti-TM16A antibody (A30508). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-03
Question
I see that the anti-TM16A antibody A30508 works with IF, what is the protocol used to produce the result images on the product page?
E. Collins
Verified customer
Asked: 2016-02-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-02-18