Product Info Summary
SKU: | A01596 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MRP4/ABCC4 Antibody Picoband®
View all MRP4/ABCC4 Antibodies
SKU/Catalog Number
A01596
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MRP4/ABCC4 Antibody Picoband® catalog # A01596. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP4/ABCC4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01596)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MRP4 recombinant protein (Position: M1-K77).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01596 is reactive to ABCC4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200-250 kDa
Calculated molecular weight
149527 MW
Background of MRP4/ABCC4
ABCC4 (Atp-binding cassette, subfamily c, member 4), also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene. It belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. This gene is mapped to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of ABCC4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of ABCC4 activity or knockdown of ABCC4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. It has been found that ABCC4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01596 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human SW620 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MRP4 using anti-MRP4 antibody (A01596).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP4 antigen affinity purified polyclonal antibody (Catalog # A01596) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP4 at approximately 200-250 kDa. The expected band size for MRP4 is at 150 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ABCC4 (Source: Uniprot.org, NCBI)
Gene Name
ABCC4
Full Name
Multidrug resistance-associated protein 4
Weight
149527 MW
Superfamily
ABC transporter superfamily
Alternative Names
Multidrug resistance-associated protein 4;ATP-binding cassette sub-family C member 4;MRP/cMOAT-related ABC transporter;Multi-specific organic anion transporter B;MOAT-B;ABCC4;MRP4; ABCC4 MOAT-B, MOATB, MRP4 ATP binding cassette subfamily C member 4 multidrug resistance-associated protein 4|MRP/cMOAT-related ABC transporter|bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4)|canalicular multispecific organic anion transporter (ABC superfamily)|multi-specific organic anion transporter B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCC4, check out the ABCC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP4/ABCC4 Antibody Picoband® (A01596)
Hello CJ!
A01596 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen L,Liao L,Zhai T,Huang X,Chen Y.Influence of Orally Administered Borneol on the Expression of Hepatic Transporters in Rats.Eur J Drug Metab Pharmacokinet.2019 Feb;44(1):103-109.doi:10.1007/s13318-018-0499-1.PMID:30105488.
Species: Rat
A01596 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-MRP4/ABCC4 Antibody Picoband®
Question
I was wanting to use your anti-MRP4/ABCC4 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
As indicated on the product datasheet, A01596 anti-MRP4/ABCC4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-04
Question
Is this A01596 anti-MRP4/ABCC4 antibody reactive to the isotypes of ABCC4?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The immunogen of A01596 anti-MRP4/ABCC4 antibody is E.coli-derived human MRP4 recombinant protein (Position: M1-K77). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-13
Question
My question regarding product A01596, anti-MRP4/ABCC4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Kulkarni
Verified customer
Asked: 2019-03-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01596 anti-MRP4/ABCC4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-04
Question
Is a blocking peptide available for product anti-MRP4/ABCC4 antibody (A01596)?
L. Evans
Verified customer
Asked: 2019-01-01
Answer
We do provide the blocking peptide for product anti-MRP4/ABCC4 antibody (A01596). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-01
Question
Will anti-MRP4/ABCC4 antibody A01596 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
According to the expression profile of leukemic t-cell, ABCC4 is highly expressed in leukemic t-cell. So, it is likely that anti-MRP4/ABCC4 antibody A01596 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-12-25