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Product Info Summary
| SKU: | PB9702 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Tissue Factor/F3 Antibody Picoband®
View all Coagulation Factor III/Tissue Factor Antibodies
SKU/Catalog Number
PB9702
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tissue Factor/F3 Antibody Picoband® catalog # PB9702. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tissue Factor/F3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9702)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Tissue Factor recombinant protein (Position: A29-A294). Mouse Tissue Factor shares 62.2% and 82.1% amino acid (aa) sequence identity with human and rat Tissue Factor, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9702 is reactive to F3 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
32935 MW
Background of Coagulation Factor III/Tissue Factor
Tissue factor also called platelet tissue factor, factor III, or CD142. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9702 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Lung Tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Tissue Factor using anti-Tissue Factor antibody (PB9702).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse Lung Tissue Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tissue Factor antigen affinity purified polyclonal antibody (Catalog # PB9702) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tissue Factor at approximately 50 kDa. The expected band size for Tissue Factor is at 33 kDa.
Protein Target Info & Infographic
Gene/Protein Information For F3 (Source: Uniprot.org, NCBI)
Gene Name
F3
Full Name
Tissue factor
Weight
32935 MW
Superfamily
tissue factor family
Alternative Names
Tissue factor;TF;Coagulation factor III;CD142;F3;Cf-3, Cf3; F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F3, check out the F3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tissue Factor/F3 Antibody Picoband® (PB9702)
Hello CJ!
PB9702 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Guo Z, Qi W, Yu Y, Du S, Wu J, Liu J. Diabetol Metab Syndr. 2014 Feb 28;6(1):29. Doi: 10.1186/1758-5996-6-29. Effect Of Exenatide On The Cardiac Expression Of Adiponectin Receptor 1 And Nadph Oxidase Subunits And Heart Function In Streptozotocin-I...
Guo Z, Zhang R, Li J, Xu G. Cardiovasc Diabetol. 2012 Aug 8;11:94. Doi: 10.1186/1475-2840-11-94. Effect Of Telmisartan On The Expression Of Adiponectin Receptors And Nicotinamide Adenine Dinucleotide Phosphate Oxidase In The Heart And Aorta In Typ...
Li Y, Cao Y, Zeng Z, Liang M, Xue Y, Xi C, Zhou M, Jiang W. Sci Rep. 2015 Feb 3;5:8209. Doi: 10.1038/Srep08209. Angiotensin-Converting Enzyme 2/Angiotensin-(1-7)/Mas Axis Prevents Lipopolysaccharide-Induced Apoptosis Of Pulmonary Microvascular End...
Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...
Li X, Li M, Li Y, Quan Q, Wang J. Neural Regen Res. 2012 Dec 25;7(36):2860-6. Doi: 10.3969/J.Issn.1673-5374.2012.36.002. Cellular And Molecular Mechanisms Underlying The Action Of Ginsenoside Rg1 Against Alzheimer'S Disease.
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Tissue Factor/F3 Antibody Picoband®
Question
Does anti-Tissue Factor/F3 antibody PB9702 work on bovine WB with prostate?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Our lab technicians have not validated anti-Tissue Factor/F3 antibody PB9702 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Tissue Factor/F3 antibody PB9702 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine prostate in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-31
Question
Does PB9702 anti-Tissue Factor/F3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
As indicated on the product datasheet, PB9702 anti-Tissue Factor/F3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-26
Question
We are currently using anti-Tissue Factor/F3 antibody PB9702 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on zebrafish tissues as well?
S. Singh
Verified customer
Asked: 2018-03-06
Answer
The anti-Tissue Factor/F3 antibody (PB9702) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate using anti-Tissue Factor/F3 antibody PB9702. Let me know if you need anything else.
N. Walker
Verified customer
Asked: 2016-12-06
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-06
