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Product Info Summary
| SKU: | M04320 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody
View all Thymidylate Synthase Antibodies
SKU/Catalog Number
M04320
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (Catalog # M04320). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04320)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: TS106 + TMS715
Isotype
IgG1, kappa + IgG1, kappa
Immunogen
Recombinant human thymidylate synthase (TS106 & TMS715)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M04320 is reactive to TYMS in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
35716 MW
Background of Thymidylate Synthase
This monoclonal antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04320 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Anti-Thymidylate Synthase Monoclonal Antibody (TS106 + TMS715).
Protein Target Info & Infographic
Gene/Protein Information For TYMS (Source: Uniprot.org, NCBI)
Gene Name
TYMS
Full Name
Thymidylate synthase
Weight
35716 MW
Superfamily
thymidylate synthase family
Alternative Names
Thymidylate synthase;TS;TSase;2.1.1.45;TYMS;TS;OK/SW-cl.29; TYMS HST422, TMS, TS thymidylate synthetase thymidylate synthase|TSase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYMS, check out the TYMS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYMS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody (M04320)
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Customer Q&As
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4 Customer Q&As for Anti-Thymidylate Synthase (5-FU Resistance Marker) TYMS Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody (M04320)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We do provide the blocking peptide for product anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody (M04320). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-13
Question
I have a question about product M04320, anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04320 anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-28
Question
Is this M04320 anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody reactive to the isotypes of TYMS?
P. Moore
Verified customer
Asked: 2013-08-22
Answer
The immunogen of M04320 anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody is Recombinant human thymidylate synthase (TS106 & TMS715). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-08-22
Question
Will anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody M04320 work on monkey IHC with placenta skin?
S. Parker
Verified customer
Asked: 2013-01-11
Answer
Our lab technicians have not tested anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody M04320 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Thymidylate Synthase (5-FU Resistance Marker) Monoclonal antibody M04320 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey placenta skin in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-01-11
