Key=store_id Key=entity_id, value=47535 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A03222-3 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2018-07-26 07:06:23 Key=updated_at, value=2024-09-26 00:58:26 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-Thrombopoietin/THPO Antibody Picoband® Key=cj_search_index Key=publication_count, value=2 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1199 Key=yoast_content_score, value=60 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Thrombopoietin/THPO Antibody A03222-3 Key=meta_description, value=Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/A/0/A03222-3-Thrombopoietin-primary-antibodies-WB-testing-1.jpg Key=small_image, value=/A/0/A03222-3-Thrombopoietin-primary-antibodies-WB-testing-1.jpg Key=thumbnail, value=/A/0/A03222-3-Thrombopoietin-primary-antibodies-WB-testing-1.jpg Key=url_key, value=anti-thrombopoietin-thpo-picoband-trade-antibody-a03222-3-boster Key=url_path, value=anti-thrombopoietin-thpo-picoband-trade-antibody-a03222-3-boster Key=options_container, value=Product Info Column Key=image_label, value=Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded Key=small_image_label, value=Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded Key=thumbnail_label, value=Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Thrombopoietin Key=gene_full_name, value=thrombopoietin Key=uniprot_id, value=P40225 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=WB Key=reactivity, value=Human, Mouse, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins. Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=predicted_reactivity Key=source_company, value=Boster A03222-3 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=37836 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Adaptive Immunity, Domain Families, Epigenetics and Nuclear Signaling, Immunology, T Cells, Transcription, Zinc Finger Key=clonality, value=Polyclonal Key=swatch_image, value=/antibody/ek0517-2-ELISA-mouse-thrombopoietin-tpo-picokine-elisa-kit.jpg Key=rating_value, value=100 Key=review_count, value=4 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_facebook_image, value=/a/0/a03220-2-plagl1-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/a/0/a03220-2-plagl1-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Amino Acid Transport:A08997|PB9959^Chloride Transport:A02397^Myoclonus:A10622-2^Thrombocythemia, Essential:A01099^Transepithelial Transport:EK1998 Key=description, value=Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-Thrombopoietin/THPO Antibody Picoband® Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Key=background, value=Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. Key=gene_name, value=THPO Key=synonyms, value=Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF Key=application_details, value=Western blot, 0.1-0.5μg/ml
Key=subcellular_localization, value=Secreted. Key=tissue_specificity, value= Found mainly in the liver, kidney and skeletal muscle. Key=protein_function, value=Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Key=application_notes, value=Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of human Thrombopoietin/THPO, which shares 89.2% amino acid (aa) sequence identity with both mouse and rat Thrombopoietin/THPO. Key=purification, value=Immunogen affinity purified. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=38 kDa Key=custom_attribute_2, value=WB: human HepG2 whole cell, human Caco-2 whole cell, rat liver tissue, mouse liver tissue Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=quantity_and_stock_status Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-Thrombopoietin/THPO Antibody Picoband®
Thrombopoietin/THPO antibody
Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thrombopoietin/THPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03222-3)
A synthetic peptide corresponding to a sequence at the N-terminus of human Thrombopoietin/THPO, which shares 89.2% amino acid (aa) sequence identity with both mouse and rat Thrombopoietin/THPO.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03222-3 is reactive to THPO in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
37836 MW
Background of Thrombopoietin/THPO
Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human Caco-2 whole cell, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysate, Lane 2: human Caco-2 whole cell lysate, Lane 3: rat liver tissue lysate, Lane 4: mouse liver tissue lysate,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody (Catalog # A03222-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on THPO, check out the THPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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