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Key=updated_at, value=2024-09-26 00:58:26
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Key=name, value=Anti-Thrombopoietin/THPO Antibody Picoband®
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Key=meta_title, value=Thrombopoietin/THPO Antibody A03222-3
Key=meta_description, value=Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded
Key=small_image_label, value=Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded
Key=thumbnail_label, value=Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded
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Key=size, value=100 μg/vial
Key=protein_name, value=Thrombopoietin
Key=gene_full_name, value=thrombopoietin
Key=uniprot_id, value=P40225
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins.
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=predicted_reactivity
Key=source_company, value=Boster A03222-3
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=37836 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Adaptive Immunity, Domain Families, Epigenetics and Nuclear Signaling, Immunology, T Cells, Transcription, Zinc Finger
Key=clonality, value=Polyclonal
Key=swatch_image, value=/antibody/ek0517-2-ELISA-mouse-thrombopoietin-tpo-picokine-elisa-kit.jpg
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Key=cj_related_products, value=Amino Acid Transport:A08997|PB9959^Chloride Transport:A02397^Myoclonus:A10622-2^Thrombocythemia, Essential:A01099^Transepithelial Transport:EK1998
Key=description, value=Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Thrombopoietin/THPO Antibody Picoband®
Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
Key=gene_name, value=THPO
Key=synonyms, value=Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF
Key=application_details, value=Western blot, 0.1-0.5μg/ml

Key=subcellular_localization, value=Secreted.
Key=tissue_specificity, value= Found mainly in the liver, kidney and skeletal muscle.
Key=protein_function, value=Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Key=application_notes, value=Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of human Thrombopoietin/THPO, which shares 89.2% amino acid (aa) sequence identity with both mouse and rat Thrombopoietin/THPO.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=38 kDa
Key=custom_attribute_2, value=WB: human HepG2 whole cell, human Caco-2 whole cell, rat liver tissue, mouse liver tissue
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Anti-Thrombopoietin/THPO Antibody Picoband®

Thrombopoietin/THPO antibody

Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A03222-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Thrombopoietin/THPO Antibody Picoband®

View all Thrombopoietin/THPO Antibodies

SKU/Catalog Number

A03222-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Thrombopoietin/THPO Antibody Picoband® catalog # A03222-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Thrombopoietin/THPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03222-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Thrombopoietin/THPO, which shares 89.2% amino acid (aa) sequence identity with both mouse and rat Thrombopoietin/THPO.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03222-3 is reactive to THPO in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

38 kDa

Calculated molecular weight

37836 MW

Background of Thrombopoietin/THPO

Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A03222-3 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Positive Control

WB: human HepG2 whole cell, human Caco-2 whole cell, rat liver tissue, mouse liver tissue

Validation Images & Assay Conditions

Gene/Protein Information For THPO (Source: Uniprot.org, NCBI)

Gene Name

THPO

Full Name

Thrombopoietin

Weight

37836 MW

Superfamily

EPO/TPO family

Alternative Names

Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF THPO MGDF, MKCSF, ML, MPLLG, THCYT1, TPO thrombopoietin thrombopoietin|MPL ligand|c-mpl ligand|megakaryocyte colony-stimulating factor|megakaryocyte growth and development factor|megakaryocyte stimulating factor|myeloproliferative leukemia virus oncogene ligand|prepro-thrombopoietin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on THPO, check out the THPO Infographic

THPO infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for THPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A03222-3

100μg

$370
A03222-3-10ug

10μg sample (liquid)

$99
A03222-3-Biotin

100 μg Biotin conjugated

$570
A03222-3-Cy3

100 μg Cy3 conjugated

$570
A03222-3-Dylight488

100 μg Dylight488 conjugated

$570
A03222-3-Dylight550

100 μg Dylight550 conjugated

$570
A03222-3-Dylight594

100 μg Dylight594 conjugated

$570
A03222-3-FITC

100 μg FITC conjugated

$570
A03222-3-HRP

100 μg HRP conjugated

$570
A03222-3-APC

100 μg APC conjugated

$670
A03222-3-PE

100 μg PE conjugated

$670
A03222-3-iFluor647

100 μg iFluor647 conjugated

$670
A03222-3-carrier-free

Carrier Free

$370

More conjugates/formats

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A03222-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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