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Product Info Summary
| SKU: | A05193 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SUMO3 Antibody
SKU/Catalog Number
A05193
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SUMO3 Antibody (Catalog # A05193). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SUMO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SUMO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05193)
Host
Rabbit
Contents
SUMO3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SUMO3 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human SUMO3. The immunogen is located within the last 50 amino acids of SUMO3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody will not cross-react with JMJD1A or JMJD1B.
Reactive Species
A05193 is reactive to SUMO3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
11637 MW
Background of SUMO3
Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05193 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SUMO3 antibody can be used for detection of SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SUMO3 in mouse liver tissue lysate with SUMO3 antibody at (A) and (B) 2 μg/mL.
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Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.
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Immunofluorescence of SUMO3 in mouse liver tissue with SUMO3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SUMO3 (Source: Uniprot.org, NCBI)
Gene Name
SUMO3
Full Name
Small ubiquitin-related modifier 3
Weight
11637 MW
Superfamily
ubiquitin family
Alternative Names
SMT3A, Smt3B, SMT3H1, SUMO-3, SMT3B, Small ubiquitin-related modifier 3, SMT3 homolog 1 SUMO3 SMT3A, SMT3H1, SUMO-3, Smt3B small ubiquitin like modifier 3 small ubiquitin-related modifier 3|SMT3 suppressor of mif two 3 homolog 1|SMT3 suppressor of mif two 3 homolog 3|ubiquitin-like protein SMT3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUMO3, check out the SUMO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUMO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SUMO3 Antibody (A05193)
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