Click image to see more details
Product Info Summary
| SKU: | PA2220 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Sumo 1/SUMO1 Antibody Picoband®
SKU/Catalog Number
PA2220
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Sumo 1/SUMO1 Antibody catalog # PA2220. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sumo 1/SUMO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2220)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Sumo 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2220 is reactive to SUMO1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
91 kDa
Calculated molecular weight
11557 MW
Background of SUMO1
Small ubiquitin-related modifier 1 (SUMO1), also called SMT3C or PIC1 is a protein that in humans is encoded by the SUMO1 gene. This gene is mapped to 2q33.1. This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2220 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Spleen Tissue, Human Placenta Tissue,
Validation Images & Assay Conditions
Click image to see more details
Anti-Sumo 1 antibody, PA2220, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Protein Target Info & Infographic
Gene/Protein Information For SUMO1 (Source: Uniprot.org, NCBI)
Gene Name
SUMO1
Full Name
Small ubiquitin-related modifier 1
Weight
11557 MW
Superfamily
ubiquitin family
Alternative Names
Small ubiquitin-related modifier 1;SUMO-1;GAP-modifying protein 1;GMP1;SMT3 homolog 3;Sentrin;Ubiquitin-homology domain protein PIC1;Ubiquitin-like protein SMT3C;Smt3C;Ubiquitin-like protein UBL1;SUMO1;SMT3C, SMT3H3, UBL1;OK/SW-cl.43; SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 small ubiquitin like modifier 1 small ubiquitin-related modifier 1|GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUMO1, check out the SUMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sumo 1/SUMO1 Antibody Picoband® (PA2220)
Hello CJ!
PA2220 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Sumo 1/SUMO1 Antibody Picoband®
Question
My colleagues were happy with the WB result of your anti-Sumo 1/SUMO1 antibody. However we have seen positive staining in placenta nucleus membrane. nucleus speckle. using this antibody. Is that expected? Could you tell me where is SUMO1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
From literature, placenta does express SUMO1. Generally SUMO1 expresses in nucleus membrane. nucleus speckle. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-10
Question
We want using your anti-Sumo 1/SUMO1 antibody for positive regulation of atpase-coupled calcium transmembrane transporter activity studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thanks for your inquiry. This PA2220 anti-Sumo 1/SUMO1 antibody is validated on rat spleen tissue, tissue lysate, human placenta tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-22
Question
We have observed staining in mouse cervix carcinoma erythroleukemia. Any tips? Is anti-Sumo 1/SUMO1 antibody supposed to stain cervix carcinoma erythroleukemia positively?
N. Patel
Verified customer
Asked: 2018-05-02
Answer
Based on literature cervix carcinoma erythroleukemia does express SUMO1. Based on Uniprot.org, SUMO1 is expressed in caudate nucleus, brain, brain placenta, placenta, colon adenocarcinoma, amygdala, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-02
Question
We are currently using anti-Sumo 1/SUMO1 antibody PA2220 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
The anti-Sumo 1/SUMO1 antibody (PA2220) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-09
