Product Info Summary
SKU: | A01382-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Sphingosine kinase 2 SphK2 Antibody
View all Sphingosine Kinase 2/SPHK2 Antibodies
SKU/Catalog Number
A01382-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Sphingosine kinase 2 SphK2 Antibody catalog # A01382-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sphingosine kinase 2 SphK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01382-2)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human SphK2 around the non-phosphorylation site of T614.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01382-2 is reactive to Sphk2 in Human, Mouse, Rat
Applications
A01382-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
69217 MW
Background of Sphingosine Kinase 2/SPHK2
Mediates the Na+-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. Involved in the clearance of bile acids and organic anions from the liver.
Kirchgessner T.G., J. Biol. Chem. 274:37161-37168(1999).
Keppler D., J. Biol. Chem. 275:23161-23168(2000).
Venter J.C., Submitted (JUL-2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
![a01382 2 anti sphingosine kinase 2 sphk2 antibody wb testing 1 a01382 2 anti sphingosine kinase 2 sphk2 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01382-2-anti-sphingosine-kinase-2-sphk2-antibody-wb-testing-1.jpg)
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Figure 1. Western blotting validation for Anti-Sphingosine kinase 2 SphK2 Antibody A01382-2
Western Blot (WB) analysis of AD293 using SphK2 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
![a01382 2 anti sphingosine kinase 2 sphk2 antibody wb testing 2 a01382 2 anti sphingosine kinase 2 sphk2 antibody wb testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01382-2-anti-sphingosine-kinase-2-sphk2-antibody-wb-testing-2.jpg)
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Figure 2. Western blotting validation for Anti-Sphingosine kinase 2 SphK2 Antibody A01382-2
Western Blot (WB) analysis of 22RV1 cells using SphK2 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Sphk2 (Source: Uniprot.org, NCBI)
Gene Name
Sphk2
Full Name
Sphingosine kinase 2
Weight
69217 MW
Alternative Names
EC 2.7.1.91; SK 2; SK2; sphingosine kinase 2; sphingosine kinase type 2 isoform; SPHK2; SPK 2; SPK2 Sphk2|C76851, Sk2, Spk2|sphingosine kinase 2|sphingosine kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sphk2, check out the Sphk2 Infographic
![Sphk2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sphk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sphingosine kinase 2 SphK2 Antibody (A01382-2)
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