Sphingosine kinase 2 Antibodies

Sphingosine kinase 2 (Sphk2)

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine.

Binds phosphoinositides. .

Gene Information

Mouse
Gene Name:	Sphk2
Uniprot:	Q9JIA7
Entrez:	56632

Family

Belongs to:
No superfamily

Alternative Names

EC 2.7.1.91; SK 2; SK2; sphingosine kinase 2; sphingosine kinase type 2 isoform; SPHK2; SPK 2; SPK2

Molecular Weight

Mass (kDA):
65.618 kDA

Genomic Context

Mouse
Location:	7|7 B3
Sequence:	7;

Diseases

• Malignant Neoplasms
• Inflammation
• Neoplasms
• Malignant Neoplasm Of Breast
• Lymphopenia
• Mammary Neoplasms
• Hypoxia
• Infarction
• Malignant Tumor Of Colon
• Ischemia
• Colonic Neoplasms
• Arthritis

• Fibrosis
• Cerebrovascular Accident
• Pertussis
• Multiple Sclerosis
• Autoimmune Reaction
• Tumor Angiogenesis
• Rheumatoid Arthritis

Pathways

• Cell Proliferation
• Cell Growth
• Cell Death
• Secretion
• Localization
• Chemotaxis
• Cell Cycle
• Rna Interference
• Cell Migration
• Cell Motility
• Cell Differentiation
• Anaphylaxis
• Receptor Internalization

• Cytokine Production
• Angiogenesis
• Histone Acetylation
• Pathogenesis
• Transport
• Cell Cycle Arrest
• Sensitization

PTMs

• Phosphorylation

• Cleavage

• Acetylation

Research Areas

• Apoptosis

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Sphk2

Best Uses Of The SPHK2 Marker From Boster Bio

This article will explain the Anti-Sphingosine Kinase 2 (SPHK2) Marker from Boster Bio. It will also explain its history and application in cell-based ELISA. The information contained in this article is applicable to all scientists around the world. This article is not intended to be an endorsement from Boster Bio. It is a brief overview about this marker and its use in cell-based ELISA.

Boster's Antisphingosine Kinase2 SphK2 Marker

Antibodies against Sphingosine recognize the anti-Sphingosine Kinasen B. Its primary function is to regulate the activity of the S1P receptor. SphK2 plays a role in exosome trafficking and has numerous other cellular functions. In addition to its function in vesicular trafficking, SphK2 regulates the cargo content of exosomes released from human K562 cells. It also associates with the N–Rh–PE late exosomes. In addition, SphK2 is involved in the maturation of exosomes within HeLa cells.

The sphingosine Kinase SPHK2 is one of the two major enzymes that catalyze sphingosine phosphorylation. It is a 654 amino-amino acid polypeptide that has a predicted molecular weight of 69 kDa. The sequence identity of the human protein is 85% with orthologs from mice and rats. SPHK2 has opposite effects on cell proliferation and promotes Apoptosis. Additionally, its function is being studied in the treatment of inflammation-related diseases.

SPHK2 is also involved in the regulation of sphingolipids as well as the lifespan of human. Although its precise functions are not fully understood, it plays a crucial function in controlling aging and many of the age-related diseases. SPHK2 is associated with multiple sclerosis (MS) and diabetes.

SPHK2 is located in the cytoplasmic as well as nuclear membrane. It has pro-apoptotic effects that regulate gene expression, as well as maintaining the integrity of telomeres. SPHK2 is also present in cancer cells as well as cardiomyocytes. It binds with prohibitin 2 and regulates mitochondrial function. It regulates functions of other proteins, like the SF1P that is phosphorylated.

Boster Bio has tested the anti-Sphingosine-Kinasen-G- anti-Sphingosine Kinasen antibody for use in WB applications. It has been demonstrated that it reacts with SphK2 in Rat and Mouse cells as well as Human cells. In addition, this anti-Sphingosine Kinase 2 antibody is also cross-reactive with Sphingosine.

SphK2 activity is decreased when DMS is added to cells at a concentration of 100 mM. When treated with lower levels of sphingosine SphK2 activity was inhibited. This is consistent with SphK2-selective activation. It is utilized in clinical trials to evaluate SphK activity in inflammatory diseases.

It is used in cell-based ELISA

Utilizing a proprietary method, the SPHK2 marker from Boster Bio can detect protein levels in living cells. It is perfect to monitor post-translational changes using in cell-based ELISA. Its simplicity along with its high sensitivity and adaptability make it a good choice for researchers. The product was created by Steven Boster, the company is today one of the biggest antibody catalogs in China. The company makes use of its own trade secrets and cutting-edge technology to offer extremely precise and sensitive ELISA kits.

The density of seeding on microplates used for assays is dependent on the type of cell size, the size of the cell, and the amount of the target protein. HeLa cells, for example, are usually seeded between 25 and 50k cells per well. Experimentation is the most effective method to determine the amount of media to add to each well. However an insufficient seeding rate will result in weak signals. Working at the top portion of this range will allow detection of very small changes in signal.

Its history

The anti-inflammatory properties of the SPHK2 gene product have been extensively studied. Although there are conflicting reports regarding its catalytic activity and its role, it is believed it regulates inflammation in macrophages of the human body. Additionally that the gene is ininversely associated with the production of inflammatory cytokine. Understanding the function of the gene in the body is crucial in this regard. This review will explore the background of the SPHK2 gene product.

The SPHK2 gene product is an isoenzyme that is linked to histone H3. It regulates acetylation histones by inhibiting the activity of histone desacetylases. This prevents them from removing acetyl group from lysine residues on histone tails. Studies have previously shown that SPHK2 is associated with the promoters of C-fos, specifically p21. More research is required to unravel the mechanism of SPHK2 regulation.

The previous research has revealed that SPHK2 is an oncogene in lung cancer. The inhibition of SPHK2 led to cell apoptosis. SPHK2-generating SP1P also binds to human Telomerase reverse transcriptase (HSCLC) cells, promoting the proliferation of cells and the growth of tumors. Furthermore, SPHK2 levels in tumors were significantly associated with lymph node status, clinical stage and histology grade.

The expression of SPHK2 in human macrophages is determined by Western blotting. Three independent experiments were conducted on the same cell line. A representative Western blot was taken for each of the samples, and p-values were determined using a one-sample t-test. However, it isn't clear whether SPHK2 plays an important role in inflammatory processes within the body. The gene is also involved in the production of inflammation-related cells.

Research has demonstrated that blocking S1P metabolism in cancer cells can increase the effectiveness of chemotherapeutic drugs. By targeting S1P metabolism enzymes can boost the effectiveness of chemotherapeutic agents or radiation. It can also help in the choice of treatment in NSCLC patients. Combining SPHK1 inhibitors with SGPL1 activators may be a novel therapeutic approach in chemoresistant NSCLC patients.

In clinical studies, SPHK2 as well as SGPL1 the levels of mRNAs are associated with survival and progression among patients with NSCLC. The impact of mRNA on prognosis is still unknown. However, high levels of SGPL1 transcripts have been associated with a higher survival rate and improved resistance to radiotherapy and chemotherapy among NSCLC patients. This study is part Jiangsu Provincial Key Medical Discipline.