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Product Info Summary
| SKU: | A01382-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human SPHK2 DyLight® 550 conjugated Antibody
View all Sphingosine Kinase 2/SPHK2 Antibodies
SKU/Catalog Number
A01382-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human SPHK2 DyLight® 550 conjugated Antibody catalog # A01382-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human SPHK2 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01382-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human SPHK2 recombinant protein (Position: F215-M269).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01382-Dyl550 is reactive to Sphk2 in Human
Applications
A01382-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
Background of Sphingosine Kinase 2/SPHK2
Sphingosine kinase 2 is a protein that in humans is encoded by the SPHK2 gene. This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For Sphk2 (Source: Uniprot.org, NCBI)
Gene Name
Sphk2
Full Name
Sphingosine kinase 2
Weight
Alternative Names
EC 2.7.1.91; SK 2; SK2; sphingosine kinase 2; sphingosine kinase type 2 isoform; SPHK2; SPK 2; SPK2 Sphk2|C76851, Sk2, Spk2|sphingosine kinase 2|sphingosine kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sphk2, check out the Sphk2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sphk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human SPHK2 DyLight® 550 conjugated Antibody (A01382-Dyl550)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Human SPHK2 DyLight® 550 conjugated Antibody
Question
We are currently using anti-Human SPHK2 DyLight® 550 conjugated antibody A01382-Dyl550 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
E. Collins
Verified customer
Asked: 2020-03-04
Answer
The anti-Human SPHK2 DyLight® 550 conjugated antibody (A01382-Dyl550) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
Is a blocking peptide available for product anti-Human SPHK2 DyLight® 550 conjugated antibody (A01382-Dyl550)?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
We do provide the blocking peptide for product anti-Human SPHK2 DyLight® 550 conjugated antibody (A01382-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-06
Question
I was wanting to use your anti-Human SPHK2 DyLight® 550 conjugated antibody for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
You can see on the product datasheet, A01382-Dyl550 anti-Human SPHK2 DyLight® 550 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Will anti-Human SPHK2 DyLight® 550 conjugated antibody A01382-Dyl550 work on monkey Flow Cytometry with liver?
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
Our lab technicians have not tested anti-Human SPHK2 DyLight® 550 conjugated antibody A01382-Dyl550 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Human SPHK2 DyLight® 550 conjugated antibody A01382-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-21
Question
I see that the anti-Human SPHK2 DyLight® 550 conjugated antibody A01382-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
B. Krishna
Verified customer
Asked: 2013-06-27
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-06-27
