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Product Info Summary
| SKU: | A02306-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MC2 receptor/MC2R Antibody Picoband®
View all Melanocortin-2 R/MC2R Antibodies
SKU/Catalog Number
A02306-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MC2 receptor/MC2R Antibody Picoband® catalog # A02306-1. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MC2 receptor/MC2R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02306-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MC2 receptor, different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02306-1 is reactive to MC2R in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
33927 MW
Background of Melanocortin-2 R/MC2R
Melanocortin-2 receptor (MC2R), also known as ACTH receptor (ACTHR), is a member of the G protein-coupled receptor family. This gene is mapped to 18p11.2. MC2R is selectively activated by adrenocorticotropic hormone, whereas the other four melanocortin receptors recognize a variety of melanocortin ligands. Mutations in MC2R can result in familial glucocorticoid deficiency. Alternate transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02306-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Human
Positive Control
WB: mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MC2 receptor using anti-MC2 receptor antibody (A02306-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC2 receptor antigen affinity purified polyclonal antibody (Catalog # A02306-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC2 receptor at approximately 39KD. The expected band size for MC2 receptor is at 34KD.
Protein Target Info & Infographic
Gene/Protein Information For MC2R (Source: Uniprot.org, NCBI)
Gene Name
MC2R
Full Name
Adrenocorticotropic hormone receptor
Weight
33927 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Adrenocorticotropic hormone receptor;ACTH receptor;ACTH-R;Adrenocorticotropin receptor;Melanocortin receptor 2;MC2-R;MC2R;ACTHR; MC2R ACTHR melanocortin 2 receptor adrenocorticotropic hormone receptor|ACTH receptor|MC2 receptor|adrenocorticotropin receptor|corticotropin receptor|melanocortin 2 receptor (adrenocorticotropic hormone)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MC2R, check out the MC2R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MC2R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MC2 receptor/MC2R Antibody Picoband® (A02306-1)
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Customer Q&As
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1 Customer Q&As for Anti-MC2 receptor/MC2R Antibody Picoband®
Question
We are currently using anti-MC2 receptor/MC2R antibody A02306-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The anti-MC2 receptor/MC2R antibody (A02306-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-05
