Product Info Summary
SKU: | M04104-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-SPG7 Mouse Monoclonal Antibody [Clone ID: OTI1C1]
SKU/Catalog Number
M04104-1
Size
100 µl
Description
Boster Bio SPG7 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1). Catalog# M04104-1. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SPG7 Mouse Monoclonal Antibody [Clone ID: OTI1C1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04104-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1C1
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7 (NP_003110) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04104-1 is reactive to SPG7 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
88.235kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04104-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SPG7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-SPG7 mouse monoclonal antibody (M04104-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
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Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody (M04104-1).
Protein Target Info & Infographic
Gene/Protein Information For SPG7 (Source: Uniprot.org, NCBI)
Gene Name
SPG7
Full Name
Paraplegin
Weight
88.235kDa
Alternative Names
CARMGC126331; cell matrix adhesion regulator; CMARMGC126332; EC 3.4.24; EC 3.4.24.-; FLJ37308; paraplegin; PGNcell adhesion regulator; spastic paraplegia 7 (pure and complicated autosomal recessive); Spastic paraplegia 7 protein; SPG5C SPG7 CAR, CMAR, PGN, SPG5C SPG7 matrix AAA peptidase subunit, paraplegin paraplegin|SPG7, paraplegin matrix AAA peptidase subunit|cell matrix adhesion regulator|spastic paraplegia 7 (pure and complicated autosomal recessive)|spastic paraplegia 7 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPG7, check out the SPG7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPG7 Mouse Monoclonal Antibody [Clone ID: OTI1C1] (M04104-1)
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