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Product Info Summary
| SKU: | M05259 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Caspase 5 CASP5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05259
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caspase 5 CASP5 Rabbit Monoclonal Antibody catalog # M05259. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase 5 CASP5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05259)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GOI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caspase 5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05259 is reactive to CASP5 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
49736 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05259 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Western blot analysis of Caspase 5 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Caspase 5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CASP5 (Source: Uniprot.org, NCBI)
Gene Name
CASP5
Full Name
Caspase-5
Weight
49736 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-5;CASP-5;3.4.22.58;ICE (rel)-III;Protease ICH-3;Protease TY;Caspase-5 subunit p20;Caspase-5 subunit p10;CASP5;ICH3; CASP5 ICE(rel)III, ICEREL-III, ICH-3 caspase 5 caspase-5|TY protease|caspase 5 variant X1|caspase 5, apoptosis-related cysteine peptidase|caspase 5, apoptosis-related cysteine protease|protease ICH-3|protease TY
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP5, check out the CASP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase 5 CASP5 Rabbit Monoclonal Antibody (M05259)
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No publications found for M05259
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Caspase 5 CASP5 Rabbit Monoclonal Antibody
Question
We are currently using anti-Caspase 5 Rabbit Monoclonal antibody M05259 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The anti-Caspase 5 Rabbit Monoclonal antibody (M05259) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-16
