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Product Info Summary
| SKU: | M00461 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Mitofusin 2 MFN2 Rabbit Monoclonal Antibody
View all Mitofusin 2 Antibodies
SKU/Catalog Number
M00461
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Mitofusin 2 MFN2 Rabbit Monoclonal Antibody catalog # M00461. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mitofusin 2 MFN2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00461)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOCA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Mitofusin 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00461 is reactive to MFN2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
86402 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00461 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human 293T whole cell, human Raji whole cell, human COLO 320 whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue
IHC: human breast carcinoma tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MFN2 using anti-MFN2 antibody (M00461).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human COLO 320 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFN2 antigen affinity purified monoclonal antibody (Catalog # M00461) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFN2 at approximately 80 kDa. The expected band size for MFN2 is at 86 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Mitofusin 2 Antibody.
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Immunofluorescent analysis of Hela cells, using Mitofusin 2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MFN2 (Source: Uniprot.org, NCBI)
Gene Name
MFN2
Full Name
Mitofusin-2
Weight
86402 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Mitofusin-2;3.6.5.-;Transmembrane GTPase MFN2;MFN2;CPRP1, KIAA0214; MFN2 CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF mitofusin 2 mitofusin-2|hyperplasia suppressor|mitochondrial assembly regulatory factor|transmembrane GTPase MFN2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MFN2, check out the MFN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MFN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mitofusin 2 MFN2 Rabbit Monoclonal Antibody (M00461)
Hello CJ!
M00461 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiao Z,Wu Y,Qu S.Fenpropathrin induces degeneration of dopaminergic neurons via disruption of the mitochondrial quality control system.Cell Death Discov.2020 Aug 25;6:78.doi:10.1038/s41420-020-00313-y.PMID:32884840;PMCID:PMC7447795.
Species: Mouse
M00461 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA
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Customer Q&As
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17 Customer Q&As for Anti-Mitofusin 2 MFN2 Rabbit Monoclonal Antibody
Question
Please see the WB image, lot number and protocol we used for pancreas using anti-Mitofusin 2 Rabbit Monoclonal antibody M00461. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-21
Question
Will M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
As indicated on the product datasheet, M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-01
Question
I would like using your anti-Mitofusin 2 Rabbit Monoclonal antibody for mitochondrion localization studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We appreciate your inquiry. This M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody is validated on hela cell lysate, mouse kidney. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-03
Question
I have a question about product M00461, anti-Mitofusin 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
My colleagues were satisfied with the WB result of your anti-Mitofusin 2 Rabbit Monoclonal antibody. However we have observed positive staining in testis mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is MFN2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Based on literature, testis does express MFN2. Generally MFN2 expresses in mitochondrion outer membrane. Regarding which tissues have MFN2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9039502
Brain, Pubmed ID: 14702039
Pancreas, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-11-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-Mitofusin 2 Rabbit Monoclonal antibody M00461. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
Is this M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody reactive to the isotypes of MFN2?
C. Carter
Verified customer
Asked: 2019-11-01
Answer
The immunogen of M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody is A synthesized peptide derived from human Mitofusin 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The anti-Mitofusin 2 Rabbit Monoclonal antibody (M00461) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-14
Question
Will anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 work for WB with pancreas?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
According to the expression profile of pancreas, MFN2 is highly expressed in pancreas. So, it is likely that anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 will work for WB with pancreas.
Boster Scientific Support
Answered: 2019-08-07
Question
Is a blocking peptide available for product anti-Mitofusin 2 Rabbit Monoclonal antibody (M00461)?
K. Banerjee
Verified customer
Asked: 2019-06-18
Answer
We do provide the blocking peptide for product anti-Mitofusin 2 Rabbit Monoclonal antibody (M00461). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-18
Question
I was wanting to use your anti-Mitofusin 2 Rabbit Monoclonal antibody for WB for mouse pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse pancreas identification?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
You can see on the product datasheet, M00461 anti-Mitofusin 2 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-01
Question
I am looking for to test anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 on mouse pancreas for research purposes, then I may be interested in using anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
The products we sell, including anti-Mitofusin 2 Rabbit Monoclonal antibody M00461, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-08
Question
Is there a BSA free version of anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 available?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-11
Question
We purchased anti-Mitofusin 2 Rabbit Monoclonal antibody for WB on brain in a previous project. I am using human, and We want to use the antibody for IF next. We want examining brain as well as testis in our next experiment. Could give a recommendation on which antibody would work the best for IF?
B. Wu
Verified customer
Asked: 2015-11-10
Answer
I took a look at the website and datasheets of our anti-Mitofusin 2 Rabbit Monoclonal antibody and it seems that M00461 has been tested on human in both WB and IF. Thus M00461 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-11-10
Question
Would anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 work on horse IF with brain?
A. Dhar
Verified customer
Asked: 2015-08-05
Answer
Our lab technicians have not tested anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Mitofusin 2 Rabbit Monoclonal antibody M00461 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-05
Question
We have observed staining in mouse testis. Do you have any suggestions? Is anti-Mitofusin 2 Rabbit Monoclonal antibody supposed to stain testis positively?
N. Krishna
Verified customer
Asked: 2013-02-22
Answer
From literature testis does express MFN2. From Uniprot.org, MFN2 is expressed in heart left ventricle, bone marrow, brain, pancreas, testis, among other tissues. Regarding which tissues have MFN2 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 9039502
Brain, Pubmed ID: 14702039
Pancreas, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2013-02-22
